GRAVITROPISM AND PHOTOTROPISM OF MAIZE COLEOPTILES - EVALUATION OF THE CHOLODNY-WENT THEORY THROUGH EFFECTS OF AUXIN APPLICATION AND DECAPITATION
GRAVITROPISM AND PHOTOTROPISM OF MAIZE COLEOPTILES - EVALUATION OF THE CHOLODNY-WENT THEORY THROUGH EFFECTS OF AUXIN APPLICATION AND DECAPITATION
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DOI：
10.1093/oxfordjournals.pcp.a078768
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发表时间：
1995-03-01
影响因子：
4.9
通讯作者：
IINO, M
中科院分区：
文献类型：
--
作者：
IINO, M
研究了玉米（Zea mays L.）胚芽鞘的行为如Cholodny-Went理论所预测的那样响应于生长素施用、去顶和这些处理的组合。向重力性是在与垂直方向成30度角时诱导的，而向光性是通过单侧蓝光脉冲诱导的。无论是向性的胚芽鞘被抑制IAA，应用作为一个环的IAA-羊毛脂膏其亚顶端部分，并通过断头。施用的IAA对完整胚芽鞘的向性和生长的影响的剂量-反应曲线以及在去头胚芽鞘中诱导的向性的时间过程可以通过文献中的三个结论来解释：（1）胚芽鞘尖端是生长素产生的部位，（2）向重力性生长素的侧向转运是沿着胚芽鞘的长度发生的，（3）向光性生长素在胚芽鞘顶端发生侧向转移。通过研究在不同的距离从顶部和IAA施加到断头胚芽鞘的切割表面断头的影响，它表明，生长素是在一个完整的胚芽鞘的顶端1毫米区和横向生长素易位向光性发生在顶端的一部分，有点超过生长素生产区。
It was investigated whether or not gravitropism and phototropism of maize (Zea mays L.) coleoptiles behave as predicted by the Cholodny-Went theory in response to auxin application, decapitation and combinations of these treatments. Gravitropism was induced at an angle of 30 degrees from the vertical, and phototropism, by a pulse of unilateral blue light. Either tropism of the coleoptile was inhibited by IAA, applied as a ring of IAA-lanolin paste to its sub-apical part, and by decapitation. The dose-response curves for the effects of applied IAA on tropisms and growth of intact coleoptiles as well as the time courses of tropisms induced in decapitated coleoptiles could be explained by the three conclusions in the literature: (1) the tip of the coleoptile is the site of auxin production, (2) lateral translocation of auxin in gravitropism occurs along the length of the coleoptile, and (3) lateral translocation of auxin in phototropism occurs in the coleoptile tip. By examining the effects of decapitation made at different distances from the top and of IAA applied to the cut surface of decapitated coleoptiles, it was indicated that auxin is produced in the apical 1 mm zone of an intact coleoptile and that lateral auxin translocation for phototropism takes place in an apical part that somewhat exceeds the zone of auxin production.