Reversible effects of fatty acids on respiration, oxidative phosphorylation, and heat production of rat liver mitochondria.
Reversible effects of fatty acids on respiration, oxidative phosphorylation, and heat production of rat liver mitochondria.
复制标题
脂肪酸对大鼠肝线粒体呼吸、氧化磷酸化和产热的可逆影响。
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
T. Nakamura
中科院分区：
文献类型：
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作者：
I. Matsuoka;T. Nakamura
1.低浓度（0--70 nmol/mg蛋白质）油酸刺激线粒体4态呼吸4倍，使每克原子耗氧呼吸的表观焓变从-112增加到-208 kJ/O，完全抑制ATP合成，对线粒体Mg-ATPase活性没有明显影响。 2. 其他脂肪酸也观察到对线粒体呼吸活动的类似影响。 3.牛血清白蛋白(BSA)保护线粒体免受油酸的影响，无论向线粒体添加油酸和BSA的顺序如何。 BSA 结合油酸的能力计算为 3.6--7.1（平均 4.9）mol 油酸/mol BSA。 4.线粒体呼吸对添加油酸或BSA的响应时间为20--25秒。
1. Oleic acid at low concentrations (0--70 nmol/mg protein) stimulated mitochondrial state 4 respiration 4-fold, increased the apparent enthalpy change of the respiration per gram atom of oxygen consumed from -112 to -208 kJ/O and completely inhibited ATP synthesis without significant effect on the Mg-ATPase activity of mitochondria. 2. Similar effects on mitochondrial respiratory activities were observed with other fatty acids. 3. Bovine serum albumin (BSA) protected mitochondria from the effects of oleic acid irrespective of the order of addition of oleic acid and BSA to mitochondria. The capacity of BSA to bind oleic acid was calculated to be 3.6--7.1 (mean, 4.9) mol of oleic acid/mol of BSA. 4. The response time of mitochondrial respiration to added oleic acid or BSA was 20--25 s.