Ruminal epithelial insulin-like growth factor-binding proteins 2, 3, and 6 are associated with epithelial cell proliferation
Ruminal epithelial insulin-like growth factor-binding proteins 2, 3, and 6 are associated with epithelial cell proliferation
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瘤胃上皮胰岛素样生长因子结合蛋白 2、3 和 6 与上皮细胞增殖相关
DOI：
10.1111/asj.13422
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发表时间：
2020
影响因子：
2
通讯作者：
S.
中科院分区：
文献类型：
--
作者：
Nishihara;K.;Suzuki;Y.;Roh;S.
本研究的目的是确定调节瘤胃上皮胰岛素样生长因子结合蛋白（IGFBP）表达的因素，并确定其在瘤胃上皮细胞增殖中的作用。原代牛瘤胃上皮细胞 (BREC) 与 pH 7.4 或 5.6 的短链脂肪酸 (SCFA)、乳酸、脂多糖 (LPS)、胰岛素样生长因子-I (IGF-I)、-II (IGF-II) 或重组牛 IGFBP2 (rbIGFBP2) 一起孵育。使用定量实时聚合酶链反应（qRT-PCR）分析 BREC 中 IGFBP 的 mRNA 表达水平。使用 WST-1 检测分析 BREC 的增殖率。SCFA 处理后 IGFBP2 基因表达趋于降低 (p< .1)，SCFA 处理后 IGFBP6 基因表达显着降低 (p< .05)。乳酸处理后 IGFBP3 和 IGFBP6 基因表达趋于较高 (p< .1)。LPS 处理时 IGFBP3 基因表达显着较高 (p< .05)。用 IGF-I 处理的 BREC 比用载体对照处理的细胞生长得更快 (p< 0.01)；然而，重组牛 rbIGFBP2 抑制 IGF-I 诱导的增殖。 IGF-II 和/或 rbIGFBP2 不影响 BREC 增殖。综上所述，SCFA处理降低了瘤胃上皮细胞中IGFBP2和IGFBP6的表达，并且这些IGFBP的低表达可能通过促进IGF-I促进瘤胃上皮细胞增殖。
The aim of this study was to identify factors that regulate ruminal epithelial insulin‐like growth factor‐binding protein (IGFBP) expression and determine its role in rumen epithelial cell proliferation. Primary bovine rumen epithelial cells (BREC) were incubated with short‐chain fatty acids (SCFAs) at pH 7.4 or 5.6, lactate, lipopolysaccharide (LPS), insulin‐like growth factor‐I (IGF‐I), ‐II (IGF‐II), or recombinant bovine IGFBP2 (rbIGFBP2). The mRNA expression levels of IGFBP in BREC were analyzed using quantitative real‐time polymerase chain reaction (qRT‐PCR). The proliferation rate of BREC was analyzed using a WST‐1 assay.IGFBP2gene expression tended to be lower with SCFA treatment (p< .1), andIGFBP6gene expression was significantly lower with SCFA treatment (p< .05).IGFBP3andIGFBP6gene expression tended to be higher withd‐Lactate treatment (p< .1).IGFBP3gene expression was significantly higher (p< .05) with LPS treatment. BREC treated with IGF‐I grew more rapidly than vehicle control‐treated cells (p< .01); however, recombinant bovine rbIGFBP2 inhibited IGF‐I‐induced proliferation. IGF‐II and/or rbIGFBP2 did not affect BREC proliferation. Taken together, SCFA treatment decreasedIGFBP2andIGFBP6expression in rumen epithelial cells, and lower expression of theseIGFBPmight promote rumen epithelial cell proliferation by facilitating IGF‐I.