Production and characterization of acidophilic xylanolytic enzymes from Penicillium oxalicum GZ-2
Production and characterization of acidophilic xylanolytic enzymes from Penicillium oxalicum GZ-2
复制标题
DOI：
10.1016/j.biortech.2012.07.051
复制
发表时间：
2012-11-01
影响因子：
11.4
通讯作者：
Xu, Yangchun
中科院分区：
文献类型：
--
作者：
Liao, Hanpeng;Xu, Chunmiao;Xu, Yangchun
草酸青霉GZ-2在麦秆、稻秆、玉米秸秆和麦麸上生长过程中产生多种嗜酸性木聚糖分解酶。木聚糖酶亚型的表达取决于底物类型和氮源。粗酶的 SDS-PAGE 分辨率产生的酶谱表明，麦秆是最好的诱导剂，在深层发酵过程中产生最高的木聚糖酶 (115.2 U/mL) 和 β-木糖苷酶 (89 mU/mL) 活性。木聚糖酶活性的最适温度和pH分别为50℃和4.0；然而，粗木聚糖酶在较宽的 pH 范围内表现出显着的稳定性，并且在 50°C、pH 4.0-8.0 下 30 分钟显示出超过 90% 的活性。结果表明，P. oxalicum GZ-2 是一种有前途的嗜酸木聚糖酶产生微生物，在生物燃料、动物饲料和食品工业应用中具有巨大的潜力。 (C) 2012 Elsevier Ltd. 保留所有权利。
Multiple acidophilic xylanolytic enzymes were produced by Penicillium oxalicum GZ-2 during growth on wheat straw, rice straw, corn stover, and wheat bran. The expression of xylanase isoforms was dependent on substrate type and nitrogen source. The zymograms produced by the SDS-PAGE resolution of the crude enzymes indicated that wheat straw was the best inducer, resulting in the highest xylanase (115.2 U/mL) and beta-xylosidase (89 mU/mL) activities during submerged fermentation. The optimum temperature and pH for xylanase activity were 50 degrees C and 4.0, respectively; however, the crude xylanase enzymes exhibited remarkable stability over a broad pH range and showed more than 90% activity at 50 degrees C for 30 min at pH 4.0-8.0. The results revealed that P. oxalicum GZ-2 is a promising acidophilic xylanase-producing microorganism that has great potential to be used in biofuels, animal feed, and food industry applications. (C) 2012 Elsevier Ltd. All rights reserved.