U2 RNA SHARES A STRUCTURAL DOMAIN WITH U1, U4, AND U5 RNAS
U2 RNA SHARES A STRUCTURAL DOMAIN WITH U1, U4, AND U5 RNAS
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DOI：
10.1002/j.1460-2075.1982.tb00022.x
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发表时间：
1982-01-01
期刊：
影响因子：
11.4
通讯作者：
JACOB, M
中科院分区：
文献类型：
--
作者：
BRANLANT, C;KROL, A;JACOB, M
之前报道过 U1、U4 和 U5 RNA 3'' 末端区域的共同结构特征。为了检查这些特征是否也存在于 U2 RNA 中，我们检查了鸡、雉鸡和大鼠 U2 RNA 3'' 末端区域的一级和二级结构。虽然野鸡和鸡之间没有观察到差异，但鸡和大鼠的序列只有 82.5% 同源。这种分歧允许提出基于最大碱基配对和二级结构保护的独特二级结构模型。从用各种核酸酶有限消化 U2 RNA 的结果中获得了相同的模型。将此结构与 U1、U4 和 U5 RNA 的结构进行比较表明，4 个 RNA 共享一个称为结构域 A 的共同结构，并由一个游离单链区域组成，其序列为 Pu-A-(U)n-G-Pup，两侧有 2 个发夹。 3''一侧的发夹非常稳定，并且环中具有Py-N-Py-Gp序列。该共同结构域的存在将结合 U RNA 和共同蛋白质结合位点之间的关系进行讨论。
Common structural features within the 3''-terminal regions of U1, U4, and U5 RNA were previously reported. To check whether these features also exist in U2 RNA, the primary and secondary structures of the 3''-terminal regions of chicken, pheasant and rat U2 RNA were examined. Whereas no difference was observed between pheasant and chicken, the chicken and rat sequences were only 82.5% homologous. Such divergence allowed a proposal for a unique model of secondary structure based on maximum base-pairing and secondary structure conservation. The same model was obtained from the results of limited digestion of U2 RNA with various nucleases. Comparison of this structure with those of U1, U4 and U5 RNA shows that the 4 RNA share a common structure designated as domain A, and consisting of a free single-stranded region with the sequence Pu-A-(U)n-G-Pup flanked by 2 hairpins. The hairpin on the 3'' side is very stable and has the sequence Py-N-Py-Gp in the loop. The presence of this common domain is discussed in connection with relationships among U RNA and common protein binding sites.