Identification and determination of geniposide contained in Gardenia jasminoides and in two preparations of mixed traditional Chinese medicines
Identification and determination of geniposide contained in Gardenia jasminoides and in two preparations of mixed traditional Chinese medicines
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DOI：
10.1016/s0021-9673(02)00365-5
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发表时间：
2002-06-28
影响因子：
4.1
通讯作者：
Tsai, TH
中科院分区：
文献类型：
--
作者：
Tsai, TR;Tseng, TY;Tsai, TH
采用高效液相色谱法，以乙腈-甲醇-5 mM磷酸二氢钠（pH4.6）（5：15：80，v/v/v）为移动的流动相，测定了栀子及中药制剂中栀子苷的含量。在0.1 - 50 μ g/ml范围内，分析的批内和批间准确度和精密度小于或等于10%。通过保留时间、加标真实标准品、改变检测波长和改变移动的相组成，确定药材及其制剂中是否存在京尼平苷。栀子果实中京尼平苷的含量。grandiflora Nakai高于Gardenia jasminoides Ellis。传统中草药制剂黄连解毒汤（66.27+/-1.98 mg/g）和陈仁厚汤（68.54+/-2.62 mg/g）中京尼平苷的浓度低于草药栀子（73.44+/-2.62 mg/g）本身。(C)2002 Elsevier Science BV保留所有权利。
A high-performance liquid chromatographic method was applied to the determination of the geniposide concentration in Gardenia fruit and preparations of traditional Chinese medicine using a mobile phase of acetonitrile-methanol-5 mM monosodium phosphate (pH 4.6) (5:15:80, v/v/v). Intra-assay and inter-assay accuracy and precision of the analyses were less than or equal to10% in the range of 0.1 through 50 mug/ml. The presence of geniposide in the medicinal herb and its preparations was ascertained by retention time, spiking with an authentic standard, change of detection wavelength and change of the composition of the mobile phase. The concentration of geniposide in the fruit of Gardenia jasminoides Ellis var. grandiflora Nakai is higher than that in Gardenia jasminoides Ellis. The concentration of geniposide in the traditional Chinese herbal medicine preparations, Huang-Lian-Jiee-Dwu-Tang (66.27+/-1.98 mg/g) and In-Chern-Hau-Tang (68.54+/-2.62 mg/g) was less than in the herb Gardenia jasminoides Ellis (73.44+/-2.62 mg/g) itself. (C) 2002 Elsevier Science BV All rights reserved.