CYANINE DYE LABELING REAGENTS - SULFOINDOCYANINE SUCCINIMIDYL ESTERS
CYANINE DYE LABELING REAGENTS - SULFOINDOCYANINE SUCCINIMIDYL ESTERS
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DOI：
10.1021/bc00020a001
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发表时间：
1993-03-01
影响因子：
4.7
通讯作者：
WAGGONER, AS
中科院分区：
文献类型：
--
作者：
MUJUMDAR, RB;ERNST, LA;WAGGONER, AS
本文报道了一系列基于磺基吲哚菁染料的新型荧光标记试剂。我们描述了这些试剂的合成和性质。它们含有琥珀酰亚胺基酯反应基团，并且可以容易地与抗体、抗生物素蛋白、DNA、脂质、聚合物和其他含氨基的材料缀合。标记试剂是水溶性的，pH不敏感，并显示在标记条件下大大减少染料聚集。其中一种试剂Cy 3可以用488-、514-和532-nm激光线激发，并且最好用546-nm汞弧线激发。另一种是Cy 5，可以用633 nm HeNe和647 nm Kr激光线激发，这些激光线可用于许多流式细胞仪和共聚焦激光扫描显微镜。在650 nm附近发射的新激光二极管也应该是Cy 5的优良激发源。
A series of new fluorescent labeling reagents based on sulfoindocyanine dyes has been developed. We describe the synthesis and properties of these reagents. They contain succinimidyl ester reactive groups and can be readily conjugated to antibodies, avidin, DNA, lipids, polymers, and other amino-group-containing materials. The labeling reagents are water soluble, pH insensitive, and show much reduced dye aggregation under labeling conditions. One of the reagents, Cy3, can be excited with the 488-, 514- and 532-nm laser lines and is optimally excited with the 546-nm mercury arc line. Another, Cy5, can be excited with the 633-nm HeNe and 647-nm Kr laser lines available with many flow cytometers and confocal laser-scanning microscopes. New laser diodes emitting near 650 nm should also be excellent excitation sources for Cy5.