Comparative transcriptome analyses revealed different heat stress responses in high- and low-GS Brassica alboglabra sprouts
Comparative transcriptome analyses revealed different heat stress responses in high- and low-GS Brassica alboglabra sprouts
复制标题
比较转录组分析揭示了高 GS 和低 GS 甘蓝芽的不同热应激反应
DOI：
10.1186/s12864-019-5652-y
复制
发表时间：
2019-04-04
期刊：
影响因子：
4.4
通讯作者：
Wang-Pruski, Gefu
中科院分区：
文献类型：
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作者：
Guo, Rongfang;Wang, Xingru;Wang-Pruski, Gefu
背景：羽衣甘蓝（Brassica alboglabra）含有较高的营养元素和功能分子，尤其是具有抗癌和抗氧化作用的芥子油苷（GS），受环境温度影响较大。为了研究羽衣甘蓝中GS生物合成与热应激反应的联系，对高GS系（HG）、低GS系（LG）、热应激下高GS系（HGT）和热应激下低GS系（LGT）的全局转录谱进行了分析。结果：基于每个RNA测序数据的3个生物学重复，在HG vs HGT、LG vs LGT和HGT中发现3901、4062和2396个差异表达基因分别获得 vs LGT。 GO注释、KEGG通路分析和DEG综合分析表明GS生物合成与热应激反应之间存在很强的相关性。注意到热处理后，与 GS 生物合成相关的 11 个差异表达基因下调，23 个热休克转录因子和 61 个热休克蛋白上调。利用另外两个高GS含量和低GS含量的羽衣甘蓝品种翠宝和顺宝来验证GS含量与热响应的关系，结果表明，高GS含量品种比低GS含量品种更耐热，尽管两个品种在热胁迫下GS含量均显着下降。此外，HSP100/ClpB、HSP90、HSP70和sHSP在高GS和低GS品种中存在差异表达。值得注意的是，HSP90和sHSPs比其他相关基因表现出明显的早期热应激反应。结论：高GS羽衣甘蓝较高的耐热性和热胁迫下芥子油苷含量的急剧下降表明GS积累与热应激反应之间存在密切关系。结合之前关于拟南芥GS缺陷突变体TU8中HSP90在高温下低表达的报道，HSP90在高GS和低GS品种中的差异表达模式及其早期热响应表明它可能是羽衣甘蓝GS代谢和耐热性的关键调节因子。
Background:Chinese kale (Brassica alboglabra) contains high nutritional elements and functional molecules, especially anticarcinogenic and antioxidant glucosinolates (GS), which was highly affected by environment temperature. To investigate the link of GS biosynthesis with heat stress response in Chinese kale, global transcription profiles of high-GS line (HG), low-GS line (LG), high-GS line under heat stress (HGT) and low-GS line under heat stress (LGT) were analyzed.Results:Based on three biological replicates of each RNA sequencing data, 3901, 4062 and 2396 differentially expressed genes in HG vs HGT, LG vs LGT and HGT vs LGT were obtained, respectively. GO annotation, KEGG pathway analysis and a comprehensive analysis of DEGs showed a strong correlation between the GS biosynthesis and heat stress response. It was noticed that 11 differentially expressed genes tied to the GS biosynthesis were down-regulated, 23 heat shock transcription factors and 61 heat shock proteins were up-regulated upon the heat treatment. Another two Chinese kale varieties Cuibao and Shunbao with high- and low- GS content respectively, were used to validate the relationship of GS content and heat-response, and the results showed that high-GS content variety were more thermotolerant than the low-GS content one although GS significantly decreased in both varieties under heat stress. In addition, HSP100/ClpB, HSP90, HSP70 and sHSPs were differentially expressed in high- and low-GS varieties. Notably, HSP90 and sHSPs showed an obviously early response to heat stress than other related genes.Conclusion:The higher heat resistance of high-GS Chinese kale and the sharp decrease of glucosinolate content under heat stress indicated a strong relationship of GS accumulation and heat stress response. Combined with the previous report on the low expression of HSP90 at elevated temperatures in GS-deficient mutant TU8 of Arabidopsis, the differential expression pattern of HSP90 in high- and low- GS varieties and its early heat response implied it might be a key regulator in GS metabolism and heat-resistance in Chinese kale.