Detect the Hybridization of Single-Stranded DNA by Parallel Scan Spectral Surface Plasmon Resonance Imaging
Detect the Hybridization of Single-Stranded DNA by Parallel Scan Spectral Surface Plasmon Resonance Imaging
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DOI：
10.1007/s11468-013-9530-1
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发表时间：
2013-06-01
期刊：
影响因子：
3
通讯作者：
He, Yonghong
中科院分区：
文献类型：
--
作者：
Chong, Xinyuan;Liu, Le;He, Yonghong
我们研究了平行扫描光谱表面等离子体共振成像系统在分析单链DNA（ssDNA）杂交芯片上的能力。ssDNA探针被巯基修饰，从而可以固定在金膜上。通过使用互补和非互补序列进行杂交实验以确认相互作用的特异性。通过对多项式拟合法和多项式拟合与质心法相结合的方法的比较，说明了数据分析在降低噪声、提高分辨率方面的重要性。最后，结果表明，平行扫描光谱表面等离子体共振成像系统可以用于高通量分析的杂交的ssDNA。
We investigate the ability of a parallel scan spectral surface plasmon resonance imaging system in analyzing hybridization of single-stranded DNA (ssDNA) on microarray. The ssDNA probes are modified by a thiol group and thereby can be immobilized onto the gold film. Hybridization experiments are carried out by using both complementary and noncomplementary sequence to confirm the specificity of interaction. We also demonstrate that the data analysis is very important in reducing the noise and improving the resolution by comparing polynomial fitting method with the combination of polynomial fitting and centroid method. Finally, the results demonstrate that the parallel scan spectral surface plasmon resonance imaging system can be used for high-throughput analysis of the hybridization of the ssDNA.