Antioxidative/oxidative effects of strontium-doped bioactive glass as bone graft. In vivo assays in ovariectomised rats
Antioxidative/oxidative effects of strontium-doped bioactive glass as bone graft. In vivo assays in ovariectomised rats
复制标题
DOI：
10.2478/v10136-012-0009-8
复制
发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
el Feki, Abdelfattah
中科院分区：
文献类型：
--
作者：
Jebahi, Samira;Oudadesse, Hassane;el Feki, Abdelfattah
近年来，氧化应激被认为是导致骨质疏松的重要病理因素。这种现象是导致骨密度低的原因。它会改变骨质并导致骨折。发现锶通过刺激骨形成和通过抑制破骨细胞减少骨吸收来诱导成骨细胞活性。生物玻璃（BG）已被用于修复骨缺损，并与锶（BG-Sr）结合，提供了治疗这种疾病的机会。本研究探讨了BG-Sr在提高抗氧化活性和再生骨能力方面的潜在作用。在体内，BG Sr和BG植入Wistar大鼠股骨髁，并与对照组进行比较。细胞增殖在SaOS 2下显著增加120%，在EAhy 926下显著增加127%。与其他组相比，BG-Sr治疗组大鼠的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）显著升高。此外，还观察到硫代巴比妥酸反应物质（TBARS）显著减少。Ca/P比值的增加促进了进行性骨矿化。根据这些结果，BG-Sr改善细胞增殖并开发了针对ROS的抗氧化防御。组织学研究结果突出了BG-Sr在骨质疏松症治疗中的意义，并通过骨结构证实。BG Sr作为一种抗氧化应激的治疗性生物材料的开发可能成为组织工程应用的有效选择。
Recently, oxidative stress has been identified as a pivotal pathological factor inducing bone osteoporosis. This phenomenon is responsible for low bone density. It alters bone quality and generates bone fractures. Strontium is found to induce osteoblast activity by stimulating bone formation and reducing bone resorption by restraining osteoclasts. Bioglass (BG) has been used to repair bone defects, and, in combination with strontium (BG-Sr), offers an opportunity to treat this disease. This study investigated the potential role of BG-Sr in improving antioxidant activity and regenerative bone capacity, The effects of both BG-Sr and BG were tested on osteoblast SaOS2 and endothelial EAhy926 cell proliferation in vitro. In vivo, BG-Sr and BG were implanted in the femoral condyles of Wistar rats and compared to that of control groups. Cell proliferation increased significantly by 120% at SaOS2 and 127% at EAhy926. Superoxide Dismutase (SOD), Catalase (CAT) and Glutathione Peroxidase (GPx) were significantly enhanced in BG-Sr treated rats compared to other groups. Moreover, a significant decrease of thiobarbituric acid-reactive substances (TBARs) was observed. The Ca/P ratio increase improved progressive bone mineralization. According to these results, BG-Sr ameliorated cell proliferation and developed an antioxidative defense against ROS. The histological findings highlight the BG-Sr implications in the osteoporosis treatment confirmed by bone construction. The development of BG-Sr as a therapeutic biomaterial protecting against oxidative stress might make an effective choice for application in tissue engineering.