Expression of hypoxia-related tissue factors in astrocytic gliomas. A multivariate survival study with emphasis upon carbonic anhydrase IX
Expression of hypoxia-related tissue factors in astrocytic gliomas. A multivariate survival study with emphasis upon carbonic anhydrase IX
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DOI：
10.1016/j.humpath.2006.07.020
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发表时间：
2007-04-01
期刊：
影响因子：
3.3
通讯作者：
Patsouris, Efstratios
中科院分区：
文献类型：
--
作者：
Korkolopoulou, Penelope;Perdiki, Marina;Patsouris, Efstratios
碳酸酐酶IX （CAIX）是一种跨膜酶，参与二氧化碳到碳酸的可逆代谢，因此参与生理pH调节。它还参与细胞分化和增殖，在大多数正常组织中不表达。最近有人推测，缺氧诱导的缺氧诱导因子（HIF-1）通路上调是大多数人类肿瘤中CAIX过表达的原因。在本研究中，我们检测了这种酶在星形细胞起源的弥漫性胶质瘤中的表达与血管内皮生长因子（VEGF）和HIF-1 α的表达、增殖率（用Ki-67抗原评估）、微血管形态和生存率的关系。在84例病例中，61例（72.6%）表现出CAIX在膜和/或细胞质上的强烈表达，并被归为阳性。免疫反应性呈会阴性分布，并随坏死程度的增加而增加(P
Carbonic anhydrase IX (CAIX) is a transmembrane enzyme involved in the reversible metabolism of carbon dioxide to carbonic acid and, hence, in physiological pH regulation. It also participates in cellular differentiation and proliferation, its expression being absent in most normal tissues. It has been recently postulated that the hypoxia-inducible factor (HIF-1) pathway up-regulated by hypoxia accounts for CAIX overexpression in most human tumors. In the present study, we examined the expression of this enzyme in diffuse gliomas of astrocytic origin in relation to vascular endothelial growth factor (VEGF) and HIF-1 alpha expression, proliferation rate (as assessed with Ki-67 antigen), microvessel morphology, and survival. Of 84 cases analyzed, 61 cases (72.6%) displayed strong membrane and/or cytoplasmic expression of CAIX and were grouped as positive. Immunoreactivity tended to have a perinecrotic distribution and increased in parallel with the extent of necrosis (P