Effect of prenatal programming and postnatal rearing on glomerular filtration rate in adult rats.
Effect of prenatal programming and postnatal rearing on glomerular filtration rate in adult rats.
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产前规划和产后饲养对成年大鼠肾小球滤过率的影响。
DOI：
10.1152/ajprenal.00593.2014
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Baum,Michel
中科院分区：
文献类型：
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作者：
Lozano,German;Elmaghrabi,Ayah;Salley,Jordan;Siddique,Khurrum;Gattineni,Jyothsna;Baum,Michel
本研究调查了产前低蛋白饮食是否导致肾小球滤过率(GFR)降低和收缩压(BP)升高。此外，我们还考察了改变哺乳新生大鼠的出生后营养环境是否会影响成年大鼠的GFR和BP。怀孕的大鼠在怀孕的最后一半期间喂以正常的(20%)蛋白质饮食或低蛋白质饮食(6%)，直到出生，然后喂以20%的蛋白质饮食。出生前低蛋白组成年大鼠出现收缩期高血压，肾小球滤过率为0.38±0.03，20%组为0.57±0.05ml.min−1·100g体重−1(P<；0.01)。在交叉养育实验中，母亲们继续吃同样的产前饮食，直到断奶。出生第1天，6%蛋白质组与2 0%母亲交叉饲养的GFR为0.5 3±0.0 5ml·min−1·10 0g Body wt−1，与与2 0%母亲交叉饲养的2 0%组(0.45±0.0 4ml·min−1·10 0g Body wt−1)差异无统计学意义。6%~20%组的BP与20%~20%组相当。孕期饲喂20%或6%蛋白质饮食并与6%母亲杂交饲养的大鼠的后代血压升高，但与20%至20%对照组相比，GFR与体重正常化。因此，产前低蛋白饮食会导致成年后代高血压和肾小球滤过率降低，这可以通过出生后的饲养来改变。
The present study examined whether a prenatal low-protein diet programs a decrease in glomerular filtration rate (GFR) and an increase in systolic blood pressure (BP). In addition, we examined whether altering the postnatal nutritional environment of nursing neonatal rats affected GFR and BP when rats were studied as adults. Pregnant rats were fed a normal (20%) protein diet or a low-protein diet (6%) during the last half of pregnancy until birth, when rats were fed a 20% protein diet. Mature adult rats from the prenatal low-protein group had systolic hypertension and a GFR of 0.38 ± 0.03 versus 0.57 ± 0.05 ml·min−1·100 g body wt−1in the 20% group (P< 0.01). In cross-fostering experiments, mothers continued on the same prenatal diet until weaning. Prenatal 6% protein rats cross-fostered to a 20% mother onday 1of life had a GFR of 0.53 ± 0.05 ml·min−1·100 g body wt−1, which was not different than the 20% group cross-fostered to a different 20% mother (0.45 ± 0.04 ml·min−1·100 g body wt−1). BP in the 6% to 20% group was comparable with the 20% to 20% group. Offspring of rats fed either 20% or 6% protein diets during pregnancy and cross-fostered to a 6% mother had elevated BP but a comparable GFR normalized to body weight as the 20% to 20% control group. Thus, a prenatal low-protein diet causes hypertension and a reduction in GFR in mature adult offspring, which can be modified by postnatal rearing.