Changes in concentration of cerebrospinal fluid components in patients with traumatic brain injury
Changes in concentration of cerebrospinal fluid components in patients with traumatic brain injury
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DOI：
10.1016/j.brainres.2006.05.057
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发表时间：
2006-08-09
期刊：
影响因子：
2.9
通讯作者：
Mayzner-Zawadzka, Ewa
中科院分区：
文献类型：
--
作者：
Toczylowska, Beata;Chalimoniuk, Malgorzata;Mayzner-Zawadzka, Ewa
脑损伤与其他中枢神经系统疾病一样，会引起脑脊液（CSF）成分的变化。在这项研究中，使用高分辨率质子（NMR）光谱法观察和监测CSF小分子浓度（最小微摩尔浓度）的变化。22例孤立性创伤性脑损伤（TBI）患者和15例对照组被招募进行研究。在ICU开始治疗前和治疗第1、3、7和14天，通过腰椎穿刺从患者的腰椎蛛网膜下腔采集CSF样本。分析了脑脊液样品的44个NMR谱信号和NO浓度。分析表明，氨基酸和有机酸浓度在治疗过程中发生变化，大多数高于对照组。已经注意到TBI患者和对照组之间所分析的CSF组分的浓度的显著差异。乳酸转化为丙酮酸的速率增加，因为L/P比率在TBI组和对照组之间没有显着差异，而TBI患者中这两种组分的浓度显着高于对照组。瓜氨酸、精氨酸和一氧化氮浓度是分析的重点。从治疗第0天到第3天，瓜氨酸浓度的变化与NO的变化重叠，而在其余的观察日，NO浓度稳定在对照水平，而瓜氨酸浓度显著降低。(c)2006 Elsevier B.V.保留所有权利。
Brain injury, like other central nervous system pathologies, causes changes in the composition of the cerebrospinal fluid (CSF). In this study, changes in the concentration of small molecules of the CSF, which are in the minimal micromolar concentration, were observed and monitored using high-resolution proton (NMR) spectroscopy. Twenty-two patients with isolated traumatic brain injuries (TBI) and 15 patients making up the control group were recruited for the study. CSF samples were collected by lumbar puncture from the lumbar subarachnoid space in the patients just before commencement of therapy and on the first, third, seventh and fourteenth days of therapy at the ICU. Forty-four signals of the NMR spectra and NO concentration of the CSF samples were analyzed. The analysis shows that the amino acid and organic acid concentrations change during the therapy and mostly are higher than in the control group. Significant differences in concentration of the analyzed CSF components between the TBI patients and the control group have been noted. The rate of the lactate to pyruvate conversion increased because the L/P ratio showed no significant differences between the TBI group and the control group, while the concentrations of both components were significantly higher in the TBI patients than in the control group. Citrulline, arginine and nitric oxide concentrations were the focus of the analysis. Citrulline concentration changes overlapped NO changes from 0 until 3rd day of therapy, while for the remaining days of observation the NO concentration stabilized at the control level, whereas citrulline concentration significantly decreased. (c) 2006 Elsevier B.V. All rights reserved.