Expression of Strawberry Notch Family Genes During Zebrafish Embryogenesis
Expression of Strawberry Notch Family Genes During Zebrafish Embryogenesis
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DOI：
10.1002/dvdy.22287
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发表时间：
2010-06-01
影响因子：
2.5
通讯作者：
Katsuyama, Yu
中科院分区：
文献类型：
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作者：
Takano, Ai;Zochi, Riyo;Katsuyama, Yu
我们以前的研究表明，Sbno 1，一个小鼠同源的草莓切口基因在大脑发育过程中的可能作用。在这篇报告中，我们克隆了斑马鱼sbno的同源基因，并通过整体原位杂交检测了它们在胚胎发生过程中的表达模式。斑马鱼有三个sbno基因：一个sbno 1同源物和两个sbno 2同源物，sbno 2a和sbno 2b。我们观察到sbno 1和sbno 2a的表达最初是普遍存在的，并随着发育的进行逐渐在中枢神经系统中占主导地位。与其他两个基因相比，sbno 2b在非神经组织中观察到表达。SBNO 1和SBNO 2a在咽期胚胎的神经系统内的不同区域中表现出较高的表达，这表明SBNO 1和SBNO 2a在胚胎发生的后期可能具有不同的作用。总之，所观察到的基因表达模式表明sbno家族基因在脊椎动物中枢神经系统发育过程中的重要作用。发展动力学239：1789-1796，2010年。(C)2010 Wiley-Liss，Inc.
Our previous study suggested a possible role for Sbno1, a mouse homologue of strawberry notch gene during brain development. In this report, we cloned the zebrafish homologues of sbno, and examined their expression pattern during embryogenesis by whole-mount in situ hybridization. Zebrafish have three sbno genes: one Sbno1 homologue and two Sbno2 homologues, sbno2a and sbno2b. We observed that the expression of sbno1 and sbno2a was initially ubiquitous and gradually became predominant in the central nervous system as development progressed. The expression of sbno2b was observed in non-neural tissues in contrast to the other two genes. sbno1 and sbno2a exhibited higher expression in distinct regions within the nervous system of pharyngula-stage embryos, suggesting possible differing roles for sbno1 and sbno2a during later stages of embryogenesis. Together, the observed gene expression patterns suggest an important role of sbno-family genes during development of the vertebrate central nervous system. Developmental Dynamics 239:1789-1796, 2010. (C) 2010 Wiley-Liss, Inc.