ITS2 barcode for identifying the officinal rhubarb source plants from its adulterants
ITS2 barcode for identifying the officinal rhubarb source plants from its adulterants
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用于识别药用大黄来源植物及其掺假品的 ITS2 条形码
DOI：
10.1016/j.bse.2016.12.004
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发表时间：
2017-02-01
影响因子：
1.6
通讯作者：
Wang, Xu-Mei
中科院分区：
文献类型：
--
作者：
Zhou, Yun;Du, Xiao-Lei;Wang, Xu-Mei
药用大黄，为大黄的干燥根和根茎，Rh. palmatum L和Rh. tanguticum（Maxim.来自Regel）Maxim.来自Balf。为蓼科蓼属植物，是我国最著名、最重要的传统中药之一。不断增长的草药市场导致了掺假品的引入，基于形态的鉴别的困难也导致了对消费者安全的担忧。以ITS2为条形码对大黄及其混伪品进行了鉴别。药用大黄源植物与混淆品之间的最小K2 P种间距离高于药用大黄源植物内部的最大K2 P种内距离。采用邻接法（NJ）、最大简约法（MP）、最大似然法（ML）和贝叶斯法构建系统发育树时，所有大黄属植物聚为一个Glade，而蓼属和酸模属植物聚为另一个Glade。在大黄沼泽地，所有的药用大黄源植物构成一个独特的亚支，而其他大黄属物种形成其他亚支。结果表明，ITS2区域是一个更合适和准确的DNA条形码，用于区分药用大黄源植物及其混淆品。(C)2016爱思唯尔有限公司版权所有。
Officinal rhubarb, the dried root and rhizome of Rheum officinale Baill., Rh. palmatum L, and Rh. tanguticum (Maxim. ex Regel) Maxim. ex Balf. of Polygonaceae family, is one of the most well-known and important traditional Chinese medicines. The growing herbal market has led to the introduction of adulterants, and difficulties in morphological based discrimination has also resulted in concerns over consumer safety. ITS2 as a potential barcode was employed to discriminate officinal rhubarb sampled from the entire distribution range and its adulterants. The minimum K2P interspecific distance between officinal rhubarb source plants and the adulterants were higher than the maximum K2P intraspecific distance within the officinal rhubarb source plants. When the phylogenetic trees were constructed by neighbor joining (NJ), maximum parsimony (MP), maximum likelihood (ML) and Bayesian methods, all Rheum species were grouped into one Glade, while Polygonum and Rumex species were clustered in another Glade. In the Rheum Glade, all the officinal rhubarb source plants constituted a distinct subclade, while other Rheum species formed other subclades. Our results suggested that the ITS2 region is a more suitable and accurate DNA barcode for distinguishing officinal rhubarb source plants from their adulterants. (C) 2016 Elsevier Ltd. All rights reserved.