PDZRN4 acts as a suppressor of cell proliferation in human liver cancer cell lines
PDZRN4 acts as a suppressor of cell proliferation in human liver cancer cell lines
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PDZRN4 在人肝癌细胞系中充当细胞增殖的抑制剂
DOI：
10.1002/cbf.3130
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发表时间：
2015-10-01
影响因子：
3.6
通讯作者：
Han, Ze-Guang
中科院分区：
文献类型：
--
作者：
Hu, Taotao;Yang, Hong;Han, Ze-Guang
近年来有研究表明LNX 1可能是胶质瘤的抑癌基因，而我们的研究首次发现LNX家族的另一个成员PDZ结构域环指蛋白4（PDZRN 4）也可能是肝细胞癌的抑癌基因。PDZRN 4，也称为LNX 4（Numb Protein-X 4的配体），是LNX家族的成员。我们最近发现，PDZRN 4，而不是LNX 1，在HCC样本中下调，PDZRN 4在HCC进展中的作用以前没有研究过。为了解决这个问题，首先，我们评估了PDZRN 4在HCC样品和邻近非癌组织中的表达。半定量聚合酶链反应显示PDZRN 4在36例肝癌中有24例表达下调，占66.7%。此外，我们的研究表明，PDZRN 4在所有12种测试的HCC细胞系中都是沉默的。随后，基于细胞的功能分析显示，PDZRN 4的异位表达抑制HCC细胞的增殖、平板集落形成和锚定非依赖性集落形成。以上结果表明，PDZRN 4可能是一个潜在的抑癌基因，对肝癌细胞的增殖具有抑制作用，对肝癌的研究具有重要意义。版权所有（c）2015约翰威利父子有限公司
Recently, some reports show that Ligand of Numb Protein-X 1 (LNX1) could be a suppressor gene in gliomas, while our current research has firstly shown that PDZ domain containing ring finger 4 (PDZRN4), another member of LNX family, could also be a potential suppressor in hepatocellular carcinoma (HCC). PDZRN4, also named LNX4 (Ligand of Numb Protein-X 4), is a member of the LNX family. We recently found that PDZRN4, but not LNX1, was down-regulated in HCC samples, and the role of PDZRN4 in the progression of HCC had not been studied before. To address this question, firstly, we evaluated the expression of PDZRN4 in HCC samples and adjacent non-cancerous tissues. Semi-quantitative polymerase chain reaction showed that PDZRN4 was down-regulated in 24/36 (66.7%) HCC samples separately. In addition, our research shows that PDZRN4 is silenced in all of the 12 HCC cell lines tested. Subsequently, cell-based functional assay exhibited that ectopic expression of PDZRN4 inhibits the proliferation, plate colony formation and anchorage-independent colony formation of HCC cells. Collectively, our results showed that PDZRN4 might be a potential tumour suppressor gene and had anti-proliferative effect on HCC cell proliferation, which would be of great significance to the researches on HCC. Copyright (c) 2015 John Wiley & Sons, Ltd.