CRISPR/Cas9 a simple, inexpensive and effective technique for gene editing.
CRISPR/Cas9 a simple, inexpensive and effective technique for gene editing.
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CRISPR/Cas9是一种用于基因编辑的简单、廉价且有效的技术。
DOI：
10.1007/s11033-022-07442-w
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发表时间：
2022-07
影响因子：
2.8
通讯作者：
Choupina, Altino Branco
中科院分区：
文献类型：
--
作者：
Ferreira, Patrick;Choupina, Altino Branco
近年来，能够在基因组的特定位置添加、移除或改变遗传物质的工具和技术数量显著增加。其目的是了解基因组的结构、基因的功能和改进基因治疗。在这项工作中，我们打算解释CRISPR/Cas9（规则间隔短回文重复序列/CRISPR相关蛋白9）的功能，以及这种技术与先前开发的技术相比可能具有的优势，例如RNA干扰（RNAi），锌指核酸酶（ZFN）和转录激活因子样效应核酸酶（TALEN）在基因和基因组编辑中。我们将从这一发现的故事开始，然后是它在细菌适应性免疫系统中对抗噬菌体攻击的生物学功能，最后是对作用机制及其在基因编辑中的应用的描述。我们还将讨论其他具有巨大潜力的Cas酶作为Cas9的替代品用于基因组编辑。CRISPR/Cas9是一种简单、廉价、有效的基因编辑技术，在功能基因组学和表观遗传学的发展中具有多种应用。在不久的将来，这项技术将在开发用于医疗和制药过程的细胞模型、靶向治疗以及农业和环境物种的改良中具有巨大的应用。
In recent years, the number of tools and techniques that enable genetic material to be added, removed or altered at specific locations in the genome has increased significantly. The objective is to know the structure of genomes, the function of genes and improve gene therapy. In this work we intend to explain the functioning of the CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein 9) and the advantages that this technique may have compared to previously developed techniques, such as RNA interference (RNAi), Zinc Finger Nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) in gene and genome editing. We will start with the story of the discovery, then its biological function in the adaptive immune system of bacteria against bacteriophage attack, and ending with a description of the mechanism of action and its use in gene editing. We will also discuss other Cas enzymes with great potential for use in genome editing as an alternative to Cas9. CRISPR/Cas9 is a simple, inexpensive, and effective technique for gene editing with multiple applications from the development of functional genomics and epigenetics. This technique will, in the near future, have great applications in the development of cell models for use in medical and pharmaceutical processes, in targeted therapy, and improvement of agricultural and environmental species.
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影响因子：
64.8
作者：
Genovese, Pietro;Schiroli, Giulia;Escobar, Giulia;Di Tomaso, Tiziano;Firrito, Claudia;Calabria, Andrea;Moi, Davide;Mazzieri, Roberta;Bonini, Chiara;Holmes, Michael C.;Gregory, Philip D.;van der Burg, Mirjam;Gentner, Bernhard;Montini, Eugenio;Lombardo, Angelo;Naldini, Luigi
通讯作者：
Naldini, Luigi
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64.8
作者：
Anders, Carolin;Niewoehner, Ole;Duerst, Alessia;Jinek, Martin
通讯作者：
Jinek, Martin
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56.9
作者：
Jinek, Martin;Chylinski, Krzysztof;Charpentier, Emmanuelle
通讯作者：
Charpentier, Emmanuelle
影响因子：
3.2
作者：
Deveau, Helene;Barrangou, Rodolphe;Moineau, Sylvain
通讯作者：
Moineau, Sylvain
影响因子：
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作者：
Krueger, Ute;Bergauer, Tobias;Konrad, Juliane
通讯作者：
Konrad, Juliane