EMBRYONIC LETHAL MUTATION IN MOUSE COLLAGEN-I GENE CAUSES RUPTURE OF BLOOD-VESSELS AND IS ASSOCIATED WITH ERYTHROPOIETIC AND MESENCHYMAL CELL-DEATH
EMBRYONIC LETHAL MUTATION IN MOUSE COLLAGEN-I GENE CAUSES RUPTURE OF BLOOD-VESSELS AND IS ASSOCIATED WITH ERYTHROPOIETIC AND MESENCHYMAL CELL-DEATH
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DOI：
10.1016/0092-8674(84)90514-2
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
JAENISCH, R
中科院分区：
文献类型：
--
作者：
LOHLER, J;TIMPL, R;JAENISCH, R
在纯合Movl3胚胎中研究了胶原I对妊娠中期发育的作用，由于插入突变，Movl3胚胎不能合成al（I）mRNA，并且大多数在妊娠第12天至第14天之间死亡。在突变胚胎中未检测到I型胶原，而其他胶原、层粘连蛋白和纤连蛋白的分布不受影响。突变胚胎正常发育至妊娠第12天，表明胶原蛋白I在形态发生的早期阶段没有重要作用。首先在肝脏的造血细胞中检测到病理事件，然后在胚胎的其他部分中检测到间充质细胞的坏死。猝死是由主要血管破裂引起的，表明胶原蛋白I在建立循环系统的机械稳定性中起着重要作用。我们的研究结果进一步表明，在胚胎发育过程中复杂的细胞相互作用，如早期造血可能依赖于I型胶原的存在。
The role of collagen I for midgestation development was studied in homozygous Movl3 embryos, which cannot synthesize al (l) mRNA as a result of insertional mutagenesis and most of which die between day 12 and 14 of gestation. No type I collagen was detected in mutant embryos, while the distribution of other collagens, laminin, and fibronectin was not affected. Mutant embryos develop normally up to day 12 of gestation, suggesting that collagen I has no essential role in the early phase of morphogenesis. The first pathological events were detected in hemopoietic cells of the liver, followed by necroses of mesenchymal cells in other parts of the embryo. The sudden death is caused by the rupture of a major blood vessel, indicating an important role for collagen I in establishing the mechanical stability of the circulatory system. Our results furthermore suggest that complex cell interactions in embryonic development such as those in early hemopoiesis may depend on the presence of collagen type I.