A light and electron microscope study of long-term organized cultures of rat dorsal root ganglia.
A light and electron microscope study of long-term organized cultures of rat dorsal root ganglia.
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DOI：
10.1083/jcb.32.2.439
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发表时间：
1967-02
期刊：
影响因子：
--
通讯作者：
Murray MR
中科院分区：
文献类型：
--
作者：
Bunge MB;Bunge RP;Peterson ER;Murray MR
将胎鼠的背根神经节移植到胶原蛋白涂层的盖玻片上，并在 Maximow 双盖玻片组件中保存长达 3 个月。这些培养的神经节在活体状态、染色的整个封片以及 OsO4 固定和 Epon 包埋后的切片中进行研究。神经突从神经细胞体的中央簇中出现，形成丰富的束状网络，通常到达承载盖玻片的边缘。这些神经元在核和细胞质的内容和组织方面与体内的对应神经元相似。例如，它们显示为“亮”或“暗”细胞，具体取决于细胞质神经丝的数量。卫星细胞在神经元胞体周围形成完整的包膜，并且其本身到处都被基底膜覆盖。神经元-卫星细胞边界因神经元胞体产生的棘状突起而变得复杂。培养物中的神经元大小、有髓纤维直径和节间长度未达到原位神经节和周围神经的已知值中的较大值 (30)。无髓鞘和有髓神经纤维以及相关的施万细胞以及神经内膜和神经束膜成分被组织成典型的束。施万细胞与其单个有髓鞘纤维或大量无髓鞘纤维的关系以及髓鞘质的特性，例如层状间距、中轴突、施密特-兰特曼裂隙、朗飞结节和突起，模拟了体内模式。结论是，通过这种技术培养胎鼠背根神经节可促进体内构成该细胞群落的所有元素（血管成分除外）的成熟和长期维持，此外，允许这些不同的成分彼此忠实地相互关联，以产生感觉神经节组织的器官型模型。
Dorsal root ganglia from fetal rats were explanted on collagen-coated coverslips and carried in Maximow double-coverslip assemblies for periods up to 3 months. These cultured ganglia were studied in the living state, in stained whole mounts, and in sections after OsO4 fixation and Epon embedment. From the central cluster of nerve cell bodies, neurites emerge to form a rich network of fascicles which often reach the edge of the carrying coverslip. The neurons resemble their in vivo counterparts in nuclear and cytoplasmic content and organization; e.g., they appear as "light" or "dark" cells, depending on the amount of cytoplasmic neurofilaments. Satellite cells form a complete investment around the neuronal soma and are themselves everywhere covered by basement membrane. The neuron-satellite cell boundary is complicated by spinelike processes arising from the neuronal soma. Neuron size, myelinated fiber diameter, and internode length in the cultures do not reach the larger of the values known for ganglion and peripheral nerve in situ (30). Unmyelinated and myelinated nerve fibers and associated Schwann cells and endoneurial and perineurial components are organized into typical fascicles. The relationship of the Schwann cell and its single myelinated fiber or numerous unmyelinated fibers and the properties of myelin, such as lamellar spacing, mesaxons, Schmidt-Lanterman clefts, nodes of Ranvier, and protuberances, mimic the in vivo pattern. It is concluded that cultivation of fetal rat dorsal root ganglia by this technique fosters maturation and long-term maintenance of all the elements that comprise this cellular community in vivo (except vascular components) and, furthermore, allows these various components to relate faithfully to one another to produce an organotypic model of sensory ganglion tissue.