Understanding and exploiting feedback in synthetic biology
Understanding and exploiting feedback in synthetic biology
复制标题
DOI：
10.1016/j.ces.2013.02.017
复制
发表时间：
2013-11-15
影响因子：
4.7
通讯作者：
Beisel, Chase L.
中科院分区：
文献类型：
--
作者：
Afroz, Taliman;Beisel, Chase L.
合成生物学在细胞和生物体的构建中采用传统的工程概念。最基本的概念之一是反馈，其中系统的活动受到其输出的影响。反馈可以使系统具有一系列所需的特性，例如减少上升时间或表现出超灵敏的响应。反馈在自然界中也很常见，这进一步支持将反馈纳入合成生物系统。在这篇综述中，我们讨论了基因调控网络中负反馈环路和正反馈环路的共同属性，无论是单独还是组合，并描述了反馈在代谢工程、群体控制和先进生物传感器开发中的最新应用。这些例子主要来自大肠杆菌和芽殖酵母酿酒酵母的合成系统，这是合成生物学的两个主要主力。通过这次审查，我们认为生物反馈是一种强大但未得到充分利用的工具，可以促进生物系统的构建。 (C) 2013 Elsevier Ltd. 保留所有权利。
Synthetic biology employs traditional engineering concepts in the construction of cells and organisms. One of the most fundamental concepts is feedback, where the activity of a system is influenced by its output. Feedback can imbue the system with a range of desirable properties such as reducing the rise time or exhibiting an ultrasensitive response. Feedback is also commonly found in nature, further supporting the incorporation of feedback into synthetic biological systems. In this review, we discuss the common attributes of negative and positive feedback loops in gene regulatory networks, whether alone or in combination, and describe recent applications of feedback in metabolic engineering, population control, and the development of advanced biosensors. The examples principally come from synthetic systems in the bacterium Escherichia coli and in the budding yeast Saccharomyces cerevisiae, the two major workhorses of synthetic biology. Through this review, we argue that biological feedback represents a powerful yet underutilized tool that can advance the construction of biological systems. (C) 2013 Elsevier Ltd. All rights reserved.