Cell Hashing with barcoded antibodies enables multiplexing and doublet detection for single cell genomics
Cell Hashing with barcoded antibodies enables multiplexing and doublet detection for single cell genomics
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DOI：
10.1186/s13059-018-1603-1
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发表时间：
2018-12-19
期刊：
影响因子：
12.3
通讯作者：
Satija, Rahul
中科院分区：
文献类型：
--
作者：
Stoeckius, Marlon;Zheng, Shiwei;Satija, Rahul
尽管单细胞测序技术发展迅速，但样本特异性批次效应、细胞多重检测和实验成本仍然是突出的挑战。在这里，我们引入了细胞哈希，其中针对普遍表达的表面蛋白的寡标记抗体独特地标记来自不同样本的细胞，随后可以将其汇集。通过对这些标签与细胞转录组进行测序，我们可以将每个细胞分配到其原始样本，可靠地识别跨样本多重，并超负荷基于液滴的商业系统，从而显着降低成本。我们使用互补遗传方法验证了我们的方法，并演示了散列如何将单细胞多重分析的优势推广到不同的样本和实验设计。
Despite rapid developments in single cell sequencing, sample-specific batch effects, detection of cell multiplets, and experimental costs remain outstanding challenges. Here, we introduce Cell Hashing, where oligo-tagged antibodies against ubiquitously expressed surface proteins uniquely label cells from distinct samples, which can be subsequently pooled. By sequencing these tags alongside the cellular transcriptome, we can assign each cell to its original sample, robustly identify cross-sample multiplets, and super-load commercial droplet-based systems for significant cost reduction. We validate our approach using a complementary genetic approach and demonstrate how hashing can generalize the benefits of single cell multiplexing to diverse samples and experimental designs.