Wide Field-of-view Fluorescence Image Deconvolution with Aberration-estimation from Fourier Ptychography References and Links-猫眼课题宝

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J. Chung;Jinho Kim;X. Ou;R. Horstmeyer;Changhuei Yang;J.-A J W Lichtman;Nat Conchello;D. J. Stephens;V. J. Allan;A R Kherlopian;T. Song;Q. Duan;M. Neimark;M. Po;J. Gohagan;A. F. Laine;G Zheng;R. Horstmeyer;C. Yang;X Ou;G. Zheng;S Dong;K. Guo;P. Nanda;R. Shiradkar;L. Tian;X. Li;K. Ramchandran;L. Waller;A Williams;J. Chung;X. Ou;S. Rawal;Z. Ao;R. Datar;R. Cote;Fourier;High;L. A. C. Nahrstedt;Schooley;P J Sementilli;B. R. Hunt;M. Nadar;L G Leff;A. A. Leff-A.;K G Porter;Y. S. Feig;C. Liang;A. Y. Park;J. Guan;P Godard;M. Allain;V. Chamard;J. Rodenburg;A. Yang;J. Qian;A. Schirotzek;F. Maia;S. Marchesini;R Horstmeyer;R. Y. Chen;B. Ames;J. Tropp;Y Shechtman;Y. Eldar;A. Szameit;M. Segev;Z. Liu;L.-H Yeh;M. Chen;J. Zhong;M Guizar-Sicairos;J. Fienup;P Thibault;M. Dierolf;A. Menzel;O. Bunk;C. David;F. Pfeiffer;High-Resolution Scanning;X-ray;G M P Kempen;V. L. J. Vliet;G E Healey;R. Kondepudy;L Yuan;J. Sun;L. Quan;H.-Y Shum;J. Lee;Y.-S Ho;R P Perry;Hanser;M. Gustafsson;D. Agard;J. Sedat

本文提出了一种利用计算显微镜方法同时获取经过像差校正的大视场荧光图像和高分辨率相干亮场图像的方法。首先，该程序应用傅立叶相变显微镜(FPM)来恢复样本的幅度和相位，其分辨率显著超过显微镜物镜的截止空间频率。同时，获取的一组FPM明场图像中的冗余提供了一种估计显微镜像差的方法。其次，该方法获取一个像差的荧光图像，并通过与估计的像差图进行反卷积来计算提高其分辨率。实验证明，在6.2 mm×9.3 mm的视场范围内，固定的、染色的和荧光标记的HeLa细胞的明场分辨率成功地提高了4.9倍，并将荧光图像中的像差引起的误差降低了31%。为了获得最佳的去卷积，我们证明了荧光图像需要具有至少~18的信噪比。系统生物学成像技术的回顾，“BMC系统。通过傅立叶层析显微镜的定量相位成像，”OPT。FP Scope：使用手机镜头的现场便携式高分辨率显微镜，“Bied。用于傅立叶变换的多路编码照明与LED阵列显微镜，”Bied。基于过滤的循环肿瘤细胞计数和分析的层析显微镜，“J.用于宽视野显微镜的空间变化像差的特征，”Opt.分辨率荧光成像通过图案照明的傅立叶层析成像，opt.决策理论中应用于两点图像的分辨率的替代方法，“J.分析非相干成像中的超分辨率的限制，J.使用绿色荧光蛋白来监控水生环境中基因工程细菌的存活，”Appl.使用DAPI鉴定和计数水生微生物区系1，利姆诺尔。体外划痕试验：一种方便和廉价的体外细胞迁移分析方法，“NAT。低计数率相干衍射成像的噪声模型，通过二次压缩传感的部分非相干光基于稀疏性的亚波长成像，OPT。具有横向平移多样性的相位恢复：一种非线性优化方法，“Opt.用于共焦显微镜的图像恢复方法的定量比较”J.渐进式尺度间和尺度内非盲图像去卷积，“ACM Transans.高质量非盲图像去卷积与自适应正则化，”J.Vis。核苷掺入细胞核和细胞质核糖核酸的动力学，《生物物理学，生物化学，高数值孔径光学系统的位相恢复》，…

This paper presents a method to simultaneously acquire an aberration-corrected, wide field-of-view fluorescence image and a high-resolution coherent bright-field image using a computational microscopy method. First, the procedure applies Fourier ptychographic microscopy (FPM) to retrieve the amplitude and phase of a sample, at a resolution that significantly exceeds the cutoff spatial frequency of the microscope objective lens. At the same time, redundancy within the set of acquired FPM bright-field images offers a means to estimate microscope aberrations. Second, the procedure acquires an aberrated fluorescence image, and computationally improves its resolution through deconvolution with the estimated aberration map. An experimental demonstration successfully improves the bright-field resolution of fixed, stained and fluorescently tagged HeLa cells by a factor of 4.9, and reduces the error caused by aberrations in a fluorescence image by up to 31%, over a field of view of 6.2 mm by 9.3 mm. For optimal deconvolution, we show the fluorescence image needs to have a signal-to-noise ratio of at least ~18. A review of imaging techniques for systems biology, " BMC Syst. Quantitative phase imaging via Fourier ptychographic microscopy, " Opt. FPscope: a field-portable high-resolution microscope using a cellphone lens, " Biomed. Multiplexed coded illumination for Fourier Ptychography with an LED array microscope, " Biomed. ptychographic microscopy for filtration-based circulating tumor cell enumeration and analysis, " J. Characterization of spatially varying aberrations for wide field-of-view microscopy, " Opt.resolution fluorescence imaging via pattern-illuminated Fourier ptychography, " Opt. Alternative approach in decision theory as applied to the resolution of two point images, " J. Analysis of the limit to superresolution in incoherent imaging, " J. Use of green fluorescent protein to monitor survival of genetically engineered bacteria in aquatic environments, " Appl. The use of DAPI for identifying and counting aquatic microflora1, " Limnol. In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro, " Nat. Noise models for low counting rate coherent diffraction imaging, " Opt. Sparsity based sub-wavelength imaging with partially incoherent light via quadratic compressed sensing, " Opt. Phase retrieval with transverse translation diversity: a nonlinear optimization approach, " Opt. A quantitative comparison of image restoration methods for confocal microscopy, " J. Progressive inter-scale and intra-scale non-blind image deconvolution, " ACM Trans. High-quality non-blind image deconvolution with adaptive regularization, " J. Vis. Kinetics of nucleoside incorporation into nuclear and cytoplasmic RNA, " J. Biophys. Biochem. Phase retrieval for high-numerical-aperture optical systems, " …

Wide Field-of-view Fluorescence Image Deconvolution with Aberration-estimation from Fourier Ptychography References and Links

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