MONOSOMY-7 IN GRANULOCYTES AND MONOCYTES IN MYELODYSPLASTIC SYNDROME
MONOSOMY-7 IN GRANULOCYTES AND MONOCYTES IN MYELODYSPLASTIC SYNDROME
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DOI：
10.1056/nejm198702263160902
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发表时间：
1987-02-26
影响因子：
158.5
通讯作者：
DELACHAPELLE, A
中科院分区：
文献类型：
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作者：
KERE, J;RUUTU, T;DELACHAPELLE, A
一些骨髓增生异常综合征或急性非淋巴细胞白血病患者的骨髓有丝分裂中7号染色体的全部或部分单体与粒细胞功能缺陷有关。为了研究单体对哪些血细胞谱系的影响，我们在Southern blotting实验中使用了7号染色体特异的DNA探针，对来自五名患者血液中特定细胞部分的DNA进行了检测。根据有无两个不同的限制性片段长度多态等位基因判断，所有5例患者的淋巴细胞都有两条不同的7号染色体。4例患者(1号、2号、4号和5号)的粒细胞受到染色体异常的影响，1例(3号)未受影响。3例患者的趋化功能正常，4例和5例患者的趋化功能受损。3例患者中2例(2号和3号)单核细胞受到单体的影响，1例基本不受影响。因此，不同患者的粒细胞和单核细胞受到不同程度的影响。我们的结论是，成熟的血细胞来源于异常的祖细胞，在不同的骨髓增生异常综合征或急性非淋巴细胞白血病患者中，不同细胞系的参与可能存在异质性。DNA丢失和功能损伤之间存在关联。
Monosomy for all or part of chromosome 7 in bone marrow mitoses of some patients with myelodysplastic syndrome or acute nonlymphocytic leukemia has been associated with a defect in granulocyte function. To study which blood-cell lineages are affected by the monosomy, we used chromosome 7-specific DNA probes in Southern blotting experiments on DNA derived from specific cell fractions isolated from the blood of five patients. As judged by the presence or absence of two different alleles for restriction-fragment-length polymorphisms, lymphocytes of all five patients were shown to have two different chromosomes 7. Granulocytes were affected by the chromosomal abnormality in four patients (No. 1, 2, 4, and 5) and unaffected in one (No. 3). Chemotaxis was normal in Patient 3 and impaired in Patients 4 and 5. Monocytes were affected by the monosomy in two of three patients (No. 2 and 3) and mainly unaffected in one (No. 1). Thus, the granulocytes and monocytes were affected differently in different patients. We conclude that mature blood cells are derived from abnormal progenitors and that there may be heterogeneity in the involvement of different cell lineages in different patients with myelodysplastic syndrome or acute nonlymphocytic leukemia. There is an association between DNA loss and functional impairment.