Gustatory Sensing Mechanism Coding for Multiple Oviposition Stimulants in the Swallowtail Butterfly, Papilio Xuthus
Gustatory Sensing Mechanism Coding for Multiple Oviposition Stimulants in the Swallowtail Butterfly, Papilio Xuthus
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DOI：
10.1523/jneurosci.1405-12.2013
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发表时间：
2013-01-16
影响因子：
5.3
通讯作者：
Ozaki, Katsuhisa
中科院分区：
文献类型：
--
作者：
Ryuda, Masasuke;Calas-List, Delphine;Ozaki, Katsuhisa
燕尾蝶（Papilio xuthus）选择性地使用芸香科中数量有限的植物。蝴蝶通过前肢化学感受器检测叶片中的产卵刺激物，并且需要多种产卵刺激物的特定组合来在其宿主植物的叶片上产卵。在这项研究中，我们试图阐明的机制，产卵行为的调控多种产卵兴奋剂。根据化学感受器的大小以及对产卵刺激物和一般促味剂的反应，将其分为L1、L2和S三种类型。L1是更丰富的女性比男性，并优先响应产卵兴奋剂。两个L2和S是共同的两性和一般tastants作出反应。我们发现，五个产卵刺激（synetamine，水苏碱，5-羟基-N ω-甲基色胺，narirabine，和chiro-inositol）引起的尖峰从三个特定的味觉受体神经元（GRNs）在L1感受器。这三个GRNs响应的混合物的五种兴奋剂的浓度相当于在柑橘的全叶提取物中发现的，和混合物诱导产卵的水平相当于全叶提取物。我们建议，产卵触发发射的三个特定的GRNs在L1感器，编码多种产卵刺激剂的化学签名。
The swallowtail butterfly, Papilio xuthus, selectively uses a limited number of plants in the Rutaceae family. The butterfly detects oviposition stimulants in leaves through foreleg chemosensilla and requires a specific combination of multiple oviposition stimulants to lay eggs on the leaf of its host plants. In this study, we sought to elucidate the mechanism underlying the regulation of oviposition behavior by multiple oviposition stimulants. We classified chemosensilla on the tarsomere of the foreleg into three types (L1, L2, and S) according to their size and response to oviposition stimulants and general tastants. The L1 was more abundant in females than in males and responded preferentially to oviposition stimulants. Both L2 and S were common to both sexes and responded to general tastants. We found that five oviposition stimulants (synephrine, stachydrine, 5-hydroxy-N omega-methyltryptamine, narirutin, and chiro-inositol) elicited spikes from three specific gustatory receptor neurons (GRNs) within L1 sensilla. These three GRNs responded to a mixture of the five stimulants at concentrations equivalent to those found in the whole-leaf extract of citrus, and the mixture induced oviposition at levels comparable to whole-leaf extract. We propose that oviposition is triggered by the firing of three specific GRNs in L1 sensilla that encode the chemical signatures of multiple oviposition stimulants.