DIAGNOSIS OF TUBERCULOSIS BY DNA AMPLIFICATION IN CLINICAL-PRACTICE EVALUATION
DIAGNOSIS OF TUBERCULOSIS BY DNA AMPLIFICATION IN CLINICAL-PRACTICE EVALUATION
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DOI：
10.1016/0140-6736(91)90492-8
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发表时间：
1991-08-10
期刊：
影响因子：
168.9
通讯作者：
GARRIGUE, G
中科院分区：
文献类型：
--
作者：
BRISSONNOEL, A;AZNAR, C;GARRIGUE, G
各种聚合酶链反应（PCR）检测已被设计用于快速鉴定临床标本中的分枝杆菌。为了评估这种检测在常规实验室工作中的价值，将PCR获得的结果与标准微生物方法获得的结果进行了比较，用于调查分枝杆菌感染的514份标本。在两种检测中检测标本中是否存在结核分枝杆菌复合群和非典型分枝杆菌，一种基于65 kDa基因的扩增，另一种基于IS 6110插入序列。对于489份不含扩增反应抑制剂的样本，PCR结果与476份（97.4%）的细菌学和/或临床数据相关性良好。PCR结果为假阴性6例，假阳性3例，误鉴定菌株4例。用硫氰酸胍预处理样品可降低假阴性结果和含有抑制剂的样品的比例。这项研究证实了DNA扩增技术在分枝杆菌感染早期诊断中的潜力。
Various polymerase chain reaction (PCR) assays have been devised for the rapid identification of mycobacteria in clinical specimens. To assess the value of such assays in routine laboratory work the results obtained by PCR were compared with those obtained by standard microbiological methods for 514 specimens collected for investigation of mycobacterial infection. Specimens were tested for the presence of Mycobacterium tuberculosis complex and atypical mycobacteria in two assays, one based on amplification of the 65 kDa gene and the other on the IS6110 insertion sequence. For the 489 samples that did not contain inhibitors of the amplification reaction PCR findings correlated well with bacteriological and/or clinical data in 476 (97.4%). 6 PCR results turned out to be false negatives, 3 to be false positives and 4 to be mis-identification of strains. Pre-treatment of samples with guanidium thiocyanate reduced the proportion of false-negative results and of samples that contained inhibitors. This study confirms the potential of DNA amplification for early diagnosis of mycobacterial infections.