Identification of potential pathogenic viruses in patients with acute myocarditis using next-generation sequencing
Identification of potential pathogenic viruses in patients with acute myocarditis using next-generation sequencing
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DOI：
10.1002/jmv.25263
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发表时间：
2018-12-01
影响因子：
12.7
通讯作者：
Ito, Yoshinori
中科院分区：
文献类型：
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作者：
Takeuchi, Suguru;Kawada, Jun-ichi;Ito, Yoshinori
心肌炎是一种心肌炎性疾病，可导致心功能障碍和心力衰竭。虽然病毒感染被认为是心肌炎最常见的病因，但病原病毒的鉴定仍然具有挑战性。最近，下一代测序（NGS）已被应用于传染病的诊断。本研究的目的是利用NGS对心肌炎患者血清中的潜在病原微生物进行综合分析。包括12名儿童和5名成人急性心肌炎住院患者。在急性期获得血清样品并使用NGS分析以检测病原体来源的DNA和RNA。NGS在17例心肌炎患者中的7例（41%）中检测到病毒序列读数。在这些患者中，通过NGS检测到的EB病毒、人细小病毒B19、细环扭体病毒和呼吸道合胞病毒读数与各1例患者的聚合酶链反应或抗原检测结果一致。通过RNA测序从一名患者中检测到大量人pegivirus读段;然而，其对人类的致病性尚不清楚。相反，在大多数情况下，检测到的病毒衍生读段的数量很少，这些病毒的病理生理作用仍有待澄清。除正常细菌植物群外，未检测到显著的细菌或真菌读数。这些数据表明，NGS的病毒衍生的DNA和RNA的综合检测可以用于鉴定潜在的致病性病毒在心肌炎。我们评估NGS检测急性心肌炎的潜在病原体的效用。在17例病例中的7例（41%）中获得了病毒序列读数。从一名患者中检测到大量人pegivirus读段。NGS有潜力检测急性心肌炎的病原体。
Myocarditis is an inflammatory disease of the myocardium and leads to cardiac dysfunction and heart failure. Although viral infections are considered to be the most common etiology of myocarditis, the identification of the causative virus is still challenging. Recently, next-generation sequencing (NGS) has been applied in the diagnosis of infectious diseases. The aim of the current study was to comprehensively analyze potential pathogenic microorganisms using NGS in the sera of patients with myocarditis. Twelve pediatric and five adult patients hospitalized for acute myocarditis were included. Serum samples in the acute phase were obtained and analyzed using NGS to detect pathogen-derived DNA and RNA. Viral sequence reads were detected in 7 (41%) of the 17 myocarditis patients by NGS. Among these patients, detection of Epstein-Barr virus, human parvovirus B19, torque teno virus, and respiratory syncytial virus reads by NGS was consistent with polymerase chain reaction or antigen test results in one patient each. A large number of human pegivirus reads were detected from one patient by RNA sequencing; however, its pathogenicity to human is unknown. Conversely, the number of detected virus-derived reads was small in most cases, and the pathophysiological role of these viruses remains to be clarified. No significant bacterial or fungal reads other than normal bacterial flora was detected. These data indicate that comprehensive detection of virus-derived DNA and RNA using NGS can be useful for the identification of potential pathogenic viruses in myocarditis.We evaluated the utility of NGS to detect potential pathogens of acute myocarditis. Viral sequence reads were obtained in 7 of 17 (41%) cases. A large number of human pegivirus reads was detected from one patient. NGS has the potential to detect the etiologic agent of acute myocarditis.