REGULATED EXPRESSION OF HUMAN A-GAMMA-GLOBIN, BETA-GLOBIN, AND HYBRID GAMMA-BETA-GLOBIN GENES IN TRANSGENIC MICE - MANIPULATION OF THE DEVELOPMENTAL EXPRESSION PATTERNS
REGULATED EXPRESSION OF HUMAN A-GAMMA-GLOBIN, BETA-GLOBIN, AND HYBRID GAMMA-BETA-GLOBIN GENES IN TRANSGENIC MICE - MANIPULATION OF THE DEVELOPMENTAL EXPRESSION PATTERNS
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DOI：
10.1016/0092-8674(86)90862-7
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发表时间：
1986-07-04
期刊：
影响因子：
64.5
通讯作者：
GROSVELD, F
中科院分区：
文献类型：
--
作者：
KOLLIAS, G;WRIGHTON, N;GROSVELD, F
我们已将人类胎儿γ-和成人β-珠蛋白基因引入到小鼠种系中。对所得转基因小鼠的分析表明，人γ-珠蛋白基因的表达类似于胚胎小鼠珠蛋白基因；人类β-珠蛋白基因的表达（如前所示）类似于成年小鼠珠蛋白基因。这些结果意味着珠蛋白基因的组织和发育阶段特异性表达的调节信号在人和小鼠之间是保守的，但信号的时间发生了变化。由于这两个基因的表达不同，我们引入了杂合γ.β-珠蛋白基因构建体。调节序列的组合导致杂合基因在所有鼠类红细胞组织中的所有阶段表达。
We have introduced the human fetal .gamma.- and adult .beta.-globin genes into the germ line of mice. Analysis of the resulting transgenic mice shows that the human .gamma.-globin gene is expressed like an embryonic mouse globin gene; the human .beta.-globin gene is expressed (as previously shown) like an adult mouse globin gene. These results imply that the regulatory signals for tissue- and development stage-specific expression of the globin genes have been conserved between man and mouse but that the timing of the signals has changed. Because the two genes are expressed differently, we introduced a hybrid .gamma..beta.-globin gene construct. The combination of the regulatory sequences resulted in the expression of the hybrid gene at all stages in all the murine erythroid tissues.