Inhibitory Effect of Crocin Against Gastric Carcinoma via Regulating TPM4 Gene.
Inhibitory Effect of Crocin Against Gastric Carcinoma via Regulating TPM4 Gene.
复制标题
藏红花素通过调控TPM4基因抑制胃癌作用
DOI：
10.2147/ott.s254167
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发表时间：
2021
影响因子：
4
通讯作者：
Zhang C
中科院分区：
文献类型：
--
作者：
Luo Y;Yu P;Zhao J;Guo Q;Fan B;Diao Y;Jin Y;Wu J;Zhang C
Background Gastric cancer (GC) is one of the most common malignant tumors and the second most frequent cause of cancer death worldwide. Crocin is a kind of bioactive constituent found in the stigmas of saffron, which has shown various pharmacological activities. Methods In this study, we investigated the inhibitory effect of crocin on gastric cancer AGS cells proliferation and explored the underlying mechanism. A series of methods were used including cell counting kit assay, gene microarray analysis, qRT-PCR, Celigo image cytometry, cell clone formation assay, Western blot, and cell xenograft growth in vivo. Results The results indicated that crocin inhibited AGS cells proliferation and promoted cell apoptosis. Further studies suggested that crocin decreased a series of genes expression, among which TPM4 gene downregulation inhibited the tumor cells proliferation and tumor growth in mice, and overexpression of TPM4 gene abolishes the inhibitory effect of crocin. Further study using microarray analysis suggested that knocking down of TPM4 altered genes related to the proliferation and apoptosis of cells. Discussion Crocin could inhibit the gastric cancer cells AGS cells proliferation by regulating TPM4 gene expression, and TPM4 may be a promising therapeutic target for GC treatment.
