Dietary supplementation of gingerols- and shogaols-enriched ginger root extract attenuate pain-associated behaviors while modulating gut microbiota and metabolites in rats with spinal nerve ligation.
Dietary supplementation of gingerols- and shogaols-enriched ginger root extract attenuate pain-associated behaviors while modulating gut microbiota and metabolites in rats with spinal nerve ligation.
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DOI：
10.1016/j.jnutbio.2021.108904
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Neugebauer V
中科院分区：
文献类型：
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作者：
Shen CL;Wang R;Ji G;Elmassry MM;Zabet-Moghaddam M;Vellers H;Hamood AN;Gong X;Mirzaei P;Sang S;Neugebauer V
神经炎症是神经病理性疼痛（NP）的核心因素。生姜是一个有前途的生物活性化合物在NP管理由于其抗炎特性。新出现的证据表明，肠道微生物组和肠道衍生代谢物在NP中起着关键作用。我们评估了两种富含姜辣素（GEG）和姜烯酚（SEG）的生姜根提取物对NP大鼠疼痛敏感性、焦虑样行为、循环无细胞线粒体DNA（ccf-mtDNA）、肠道微生物组组成和粪便代谢物的影响。将16只雄性大鼠随机分为假手术组、脊神经结扎组、脊神经结扎+0.75%GEG组和脊神经结扎+0.75%SEG组。与SNL组相比，SNL+GEG组和SNL+SEG组的疼痛和焦虑样行为以及ccf-mtDNA水平均显著降低。相对于SNL组，SNL+GEG和SNL+SEG组均增加了乳球菌属、单胞菌属、布劳特氏菌属、丹毒梭菌科和厌氧噬菌科的相对丰度，但降低了普雷沃氏菌科UCG-001、Rikenellaceae RC 9肠群、粘螺菌属和脱硫弧菌属、脱硫弧菌属、厌氧丝状菌属、野生真杆菌属群、RF 39、UCG-005、毛螺菌科NK 4A 136群、醋酸因子、反刍真杆菌群、梭菌UCG-014和未培养的厌氧噬菌科。GEG和SEG对肠源性代谢产物的影响不同。与SNL组相比，SNL+GEG组的1 '-乙酰氧基胡椒酚乙酸酯、（4 E）-1，7-双（4-羟基苯基）-4-庚烯-3-酮，NP-000629，7，8-二甲氧基-3-（2-甲基-3-丁烯-2-基）-2H-苯并吡喃-2-酮，3-{[4-（2-甲基-3-丁烯-2-基）-2H-苯并吡喃（2-嘧啶基）哌嗪基]羰基}-2-吡嗪羧酸，920863，和（1 R，3R，7 R，13 S）-13-甲基-6-亚甲基-4，14，16-三氧杂四环[11.2.1.0 ~ 1，10 ~.0~ 3，7 ~]十六碳-9-烯-5-酮，SNL+SEG组（+/-）-5-[（叔丁基氨基）-2 '-羟基丙氧基]-1_2_3_4-四氢-1-萘酚和脱氢表雄酮硫酸酯含量较高。总之，生姜是一种很有前途的功能性食品，在管理NP，和进一步的调查是必要的，以评估生姜在肠-脑轴疼痛管理的作用。
Neuroinflammation is a central factor in neuropathic pain (NP). Ginger is a promising bioactive compound in NP management due to its anti-inflammatory property. Emerging evidence suggests that gut microbiome and gut-derived metabolites play a key role in NP. We evaluated the effects of two ginger root extracts rich in gingerols (GEG) and shogaols (SEG) on pain sensitivity, anxiety-like behaviors, circulating cell-free mitochondrial DNA (ccf-mtDNA), gut microbiome composition, and fecal metabolites in rats with NP. Sixteen male rats were divided into four groups: sham, spinal nerve ligation (SNL), SNL+0.75%GEG in diet, and SNL+0.75%SEG in diet groups for 30 days. Compared to SNL group, both SNL+GEG and SNL+SEG groups showed a significant reduction in pain- and anxiety-like behaviors, and ccf-mtDNA level. Relative to the SNL group, both SNL+GEG and SNL+SEG groups increased the relative abundance of Lactococcus, Sellimonas, Blautia, Erysipelatoclostridiaceae, and Anaerovoracaceae, but decreased that of Prevotellaceae UCG-001, Rikenellaceae RC9 gut group, Mucispirillum and Desulfovibrio, Desulfovibrio, Anaerofilum, Eubacterium siraeum group, RF39, UCG-005, Lachnospiraceae NK4A136 group, Acetatifactor, Eubacterium ruminantium group, Clostridia UCG-014, and an uncultured Anaerovoracaceae. GEG and SEG had differential effects on gut-derived metabolites. Compared to SNL group, SNL+GEG group had higher level of 1'-acetoxychavicol acetate, (4E)-1,7-Bis(4-hydroxyphenyl)-4-hepten-3-one, NP-000629, 7,8-Dimethoxy-3-(2-methyl-3-buten-2-yl)-2H-chromen-2-one, 3-{[4-(2-Pyrimidinyl)piperazino]carbonyl}-2-pyrazinecarboxylic acid, 920863, and (1R,3R,7R,13S)-13-Methyl-6-methylene-4,14,16-trioxatetracyclo[11.2.1.0~1,10~.0~3,7~]hexadec-9-en-5-one, while SNL+SEG group had higher level for (+/−)-5-[(tert-Butylamino)-2'-hydroxypropoxy]-1_2_3_4-tetrahydro-1-naphthol and dehydroepiandrosteronesulfate. In conclusion, ginger is a promising functional food in the management of NP, and further investigations are necessary to assess the role of ginger on gut-brain axis in pain management.