Reciprocal association of serum Mac-2 binding protein and HDL-cholesterol concentrations
Reciprocal association of serum Mac-2 binding protein and HDL-cholesterol concentrations
复制标题
血清 Mac-2 结合蛋白与 HDL-胆固醇浓度的相互关系
DOI：
10.1016/j.cca.2021.02.001
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发表时间：
2021
影响因子：
5
通讯作者：
Kihara Shinji
中科院分区：
文献类型：
--
作者：
Uekita Hiromi;Yamamoto Hiroyasu;Niinaga Ryu;Yamane Noriko;Yoshii Manami;Yamauchi-Takihara Keiko;Kihara Shinji
Mac-2结合蛋白(Mac-2BP)是非酒精性脂肪性肝炎的血清标志物，被认为是代谢综合征(Mets)的一种肝脏表型。在这项研究中，我们调查了体内与血清Mac-2BP浓度相关的Mets相关的临床参数，以及体外的潜在机制。材料与方法我们招募了54名在大阪大学保健中心接受健康检查的日本健康男性。所有受试者均获得生理和血清生化指标。结果单因素分析显示，血清Mac-2BP浓度与高密度脂蛋白胆固醇呈负相关，与体重指数、血压呈正相关，与γ结合盒转运蛋白A1呈正相关。这些结果提示Mac-2BP与蛋氨酸有关联，但在多因素分析中这3个参数与血清Mac-2BP浓度均无显著相关性。在γ刺激下，细胞内Mac-2BP浓度升高，ABCA1和apoA-imRNA浓度下降，而Mac-2BP对ABCA1和apoA-I浓度无影响。结论健康人血清Mac-2BP浓度与高密度脂蛋白浓度呈负相关。
BackgroundMac-2 binding protein (Mac-2BP) is used as a serum biomarker of nonalcoholic steatohepatitis, considered to be a liver phenotype of metabolic syndrome (MetS). In this study, we investigated the serum Mac-2BP concentrations-correlated MetS-related clinical parametersin vivo, and the underlying mechanismin vitro.Materials & methodsWe enrolled 54 healthy Japanese men who underwent health examination at Osaka University Health Care Center in this study. Physical and serum biochemical parameters were obtained from all the subjects. In the cultured HepG2 cells, the effects of interferon (IFN)-γ on the expression of Mac-2BP, apolipoprotein (apo) A-I, and ATP binding cassette transporter A1 (ABCA1) were studied.ResultsSerum Mac-2BP concentrations correlated negatively with HDL-C, and positively with body mass index and systolic blood pressure in univariate analysis. These results suggested the association between Mac-2BP and MetS, although none of these 3 parameters had significant correlation with serum Mac-2BP concentrations in multivariate analysis. In HepG2 cells, IFN-γ stimulation resulted in the increased Mac-2BP and the decreased ABCA1 and apo A-I mRNA concentrations, while Mac-2BP had no effects on ABCA1 and apo A-I concentrations.ConclusionsThe serum Mac-2BP concentrations are negatively correlated with HDL-C concentrations in healthy subjects, as a result of chronic inflammation.