Identification of melatonin in Trichoderma spp. and detection of melatonin content under controlled‐stress growth conditions from T. asperellum
Identification of melatonin in Trichoderma spp. and detection of melatonin content under controlled‐stress growth conditions from T. asperellum
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木霉菌中褪黑激素的鉴定以及在受控胁迫生长条件下检测黑霉中褪黑激素的含量
DOI：
10.1002/jobm.201500223
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发表时间：
2016
影响因子：
3.1
通讯作者：
Yan
中科院分区：
文献类型：
--
作者：
Tong Liu;F. Zhao;Zhen Liu;Y. Zuo;J. Hou;Yan
采用液相色谱-串联质谱法对koningii T., harzianum T., asperellum T., longibrachiatum T.和viride T.进行分析，以确定是否存在褪黑激素。结果表明，5种木霉中均含有丰富的内源性褪黑素，但培养滤液中均未发现褪黑素。褪黑素含量最高的是曲霉（27.588±0.326 μg−1干质量），其次是koningii、harzianum、longibrachiatum和viride。在控制胁迫条件下，还检测了曲霉内源褪黑素的含量。数据显示，化学应激源（CdCl2、CuSO4和H2O2）引起内源性褪黑激素水平升高。CdCl2对褪黑激素的产生有最高的刺激作用，因为产品达到达到三倍的褪黑激素含量的控制。NaCl刺激褪黑激素的减少。酸性条件（pH 3和pH 5）和微碱性条件（pH 9）使褪黑素含量增加，而pH11则使褪黑素含量显著降低，仅为12.276±0.205 μg−1干质量。本研究首次报道了木霉（Trichoderma spp）中褪黑素的含量以及不同胁迫条件下褪黑素含量的变化。
T. koningii, T. harzianum, T. asperellum, T. longibrachiatum, and T. viride were analyzed using liquid chromatography‐tandem mass spectrometry to determine whether melatonin is present. Results showed that there were abundant amounts of endogenous melatonin in five Trichoderma species, but no melatonin was found in any of the culture filtrates. T. asperellum had the highest amount of melatonin (27.588 ± 0.326 μg g−1 dry mass), followed by T. koningii, T. harzianum, T. longibrachiatum, and T. viride. The endogenous melatonin content of T. asperellum in controlled‐stress growth conditions was also detected. The data showed that chemical stressors (CdCl2, CuSO4, and H2O2) provoked an increase in endogenous melatonin levels. CdCl2 had the highest stimulatory effect on melatonin production, as the product reached reaching up to three times the melatonin content of the control. NaCl stimulated a decrease of melatonin. Acidic conditions (pH 3 and pH 5) as well as slightly alkaline conditions (pH 9) resulted in an increase in the melatonin content, whereas pH11 resulted in a significant decrease in the melatonin content, only 12.276 ± 0.205 μg g−1 dry mass. The current study is first to report melatonin content and the change of melatonin content under different stress situations in Trichoderma spp.
影响因子：
3.4
作者：
Tan, Dun-Xian;Reiter, Russel J.;Hardeland, Rudiger
通讯作者：
Hardeland, Rudiger