Aberrant expression of serum amyloid A in head and neck squamous cell carcinoma
Aberrant expression of serum amyloid A in head and neck squamous cell carcinoma
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DOI：
10.1111/j.1600-0714.2009.00777.x
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发表时间：
2010-01-01
影响因子：
3.3
通讯作者：
Ando, Yukio
中科院分区：
文献类型：
--
作者：
Shinriki, Satoru;Ueda, Mitsuharu;Ando, Yukio
血清淀粉样蛋白A（SAA）是一种急性期反应物，其血液水平通常在某些类型的肿瘤发生时升高。在这里，我们研究了基因SAA 1和蛋白SAA在头颈部鳞状细胞癌（HNSCC）和正常口腔粘膜组织中的表达，以及HNSCC患者血液SAA水平。此外，我们研究了抑制信号转导和转录激活因子3（STAT 3）信号转导对HNSCC细胞系SAS中SAA 1表达的影响。HNSCC患者血清SAA水平显著高于健康志愿者。实时定量逆转录聚合酶链反应分析显示，SAA 1在HNSCC中的表达水平明显高于正常粘膜（P < 0.0001）。Western blot和免疫组化结果显示SAA在癌组织中主要表达于肿瘤细胞，而在正常粘膜组织中未见表达。STAT 3激活的抑制剂AG 490显著降低SAS细胞中SAA 1的表达。这些数据表明SAA在HNSCC中通过Janus激酶-STAT 3途径上调。
Serum amyloid A (SAA) is an acute-phase reactant, the blood level of which is often elevated in response to some types of neoplasia. Here, we investigated expression of the gene SAA1 and the protein SAA in head and neck squamous cell carcinoma (HNSCC) and normal oral mucosal tissues as well as blood SAA levels in HNSCC patients. Also, we investigated the effects of inhibiting signal transducer and activator of transcription 3 (STAT3) signaling on SAA1 expression in the HNSCC cell line SAS. Serum SAA levels in HNSCC patients were significantly higher than those in healthy volunteers. In addition, real-time quantitative reverse transcription-polymerase chain reaction analysis revealed a significantly higher SAA1 expression level in HNSCC than in normal mucosa (P < 0.0001). Furthermore, Western blot and immunohistochemical analyzes revealed that high expression of SAA in carcinomas was detected predominantly in tumor cells, but not in normal mucosal tissues. An inhibitor of STAT3 activation, AG490, significantly reduced SAA1 expression in SAS cells. These data demonstrated that SAA was up-regulated in HNSCC through the Janus kinase-STAT3 pathway.