Angiotensin II-producing proteases from granulomatous tissue reaction in mice infected with Schistosoma mansoni.
Angiotensin II-producing proteases from granulomatous tissue reaction in mice infected with Schistosoma mansoni.
复制标题
曼氏血吸虫感染小鼠的肉芽肿组织反应产生血管紧张素 II 蛋白酶。
DOI：
10.1016/0305-0491(90)90055-x
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Epstein,WL
中科院分区：
文献类型：
--
作者：
Hirayama,K;Fukuyama,K;Epstein,WL
1.从日本血吸虫病小鼠肝肉芽肿Tris-HCl缓冲液提取液中，经凝胶过滤分离得到血管紧张素I水解酶，分别为MR 140,000和MR 70,000。两种酶具有不同的底物专一性。2.血管紧张素转换酶(ACE)的MR 140,000水解酶活性被卡托普利抑制，而马铃薯羧肽酶抑制剂对MR 70,000水解酶活性有抑制作用。3.血管紧张素I在7万mR水解酶作用下，生成一种中间体Des-Leu10-Ang-tensin I和Ang II。4.结果表明，MR 70,000酶是组织羧肽酶A，在肉芽肿性炎症中产生血管紧张素II，与ACE一样。
1. Angiotensin I hydrolases, Mr 140,000 and Mr 70,000 were separated by gel filtration from Tris-HCl buffer extract of hepatic granulomas developed in mice with schistosomiasis. Two enzymes had different substrate specificity. 2. Mr 140,000 hydrolase activity was inhibited by captopril as reported for angiotensin converting enzyme (ACE), while that of Mr 70,000 hydrolase activity was inhibited by potato carboxypeptidase inhibitor. 3. An intermediary, des-Leu10-angiotensin I and then angiotensin II were formed from angiotensin I by Mr 70,000 hydrolase. 4. The findings suggest that Mr 70,000 enzyme is tissue carboxypeptidase A, and it generates angiotensin II in granulomatous inflammation as does ACE.