The cuticle proteins: a putative role for deltamethrin resistance in Culex pipiens pallens
The cuticle proteins: a putative role for deltamethrin resistance in Culex pipiens pallens
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DOI：
10.1007/s00436-015-4683-9
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发表时间：
2015-12-01
影响因子：
2
通讯作者：
Zhu, Changliang
中科院分区：
文献类型：
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作者：
Fang, Fujin;Wang, Weijie;Zhu, Changliang
抗药性一直是一个重大的公共卫生挑战。因此，研究昆虫抗药性的机理是十分必要的。在我们以前的研究中，发现了14个基于杀虫剂抗性蛋白质组和转录组的昆虫表皮蛋白（ICP）差异积累的溴氰菊酯抗性（DR）和敏感（DS）品系的淡色库蚊。为了研究这些ICP是否与溴氰菊酯抗性相关，通过使用定量实时聚合酶链反应（qRT-PCR）在实验室和田间种群的DS和DR菌株中检测到14种ICP的不同转录水平。DS品系短期暴露于溴氰菊酯后，还测定了14种ICP的表达水平。从Cx中克隆了CpCPLCG 5基因的全长互补DNA（cDNA），该基因编码14种ICP之一。pipiens pallens与其它昆虫进行了同源性分析和系统发育分析。利用小分子干扰RNA（siRNA）技术抑制CpCPLCG 5基因的表达，研究CpCPLCG 5基因对溴氰菊酯抗性的影响。结果表明，CpCPLCG 5基因在实验室和田间群体中的表达量DR株均高于DS株，而其它13个ICP基因表达量均下调。CpCPLCG 5基因cDNA全长732 bp，开放阅读框390 bp，推导出129个氨基酸（GenBank/KF 723314，2013）。CpCPLCG 5基因敲除后，DR株的易感性增加，而其他13种ICP的表达水平升高。我们的研究结果表明，角质层蛋白与溴氰菊酯抗性在Cx。pipiens pallens
Insecticide resistance has been a major public health challenge. It is impendent to study the mechanism on insecticide resistance. In our previous study, 14 differentially accumulated insect cuticle proteins (ICPs) based on insecticide resistance proteomes and transcriptomes were found in the deltamethrin-resistant (DR) and -susceptible (DS) strains of Culex pipiens pallens. To investigate if these ICPs are associated with deltamethrin resistance, different transcriptional levels of the 14 ICPs were detected in the DS and DR strains from laboratory and field populations by using quantitative real-time polymerase chain reaction (qRT-PCR). The expression levels of the 14 ICPs were also measured after short-term exposure of the DS strain to deltamethrin. The full-length complementary DNA (cDNA) of CpCPLCG5 gene, which encodes one of the 14 ICPs, was cloned from Cx. pipiens pallens. Homology analysis and phylogenetic analysis were carried out with some other insects. Furthermore, small interfering RNA (siRNA) was used to knockdown the expression level of CpCPLCG5 gene for characterizing its contribution to deltamethrin resistance. The results showed that the expression level of CpCPLCG5 gene was higher in DR strain than in DS strain both in laboratory and field populations while the other 13 ICPs were downregulated. The full-length cDNA of CpCPLCG5 gene was 732 bp, with the ORF of 390 bp and deduced 129 amino acids (GenBank/KF723314,2013). Knockdown of CpCPLCG5 gene increased the susceptibility of the DR strain while the expression level of the other 13 ICPs elevated. Our findings indicate that the cuticle proteins are associated with deltamethrin resistance in Cx. pipiens pallens.