A Protein-Protein Interactome for an African Cichlid
A Protein-Protein Interactome for an African Cichlid
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非洲慈鲷的蛋白质-蛋白质相互作用组
DOI：
10.1145/3388440.3414916
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Ritz, Anna
中科院分区：
文献类型：
--
作者：
Preising, Gabriel A.;Faber-Hammond, Joshua J.;Renn, Suzy C.;Ritz, Anna
蛋白质-蛋白质相互作用组（PPI）已被广泛用于分类和预测与复杂表型相关的基因。PPI方法对于具有良好注释的基因组特征的生物体是成功的，但由于缺乏可用的实验数据，对于非模型生物体更具挑战性。为了应对这一挑战，实验室通过推断”interlogs”或正向同源蛋白质-蛋白质相互作用为非模型生物体创建了PPI [1]。非洲慈鲷是鱼类的一个多样化分支，已被用于研究快速进化分歧[3]。该项目提出了一种基于interlog的方法，从现有的斑马鱼相互作用组构建非洲慈鲷特异性PPI [1]。作为一个案例研究，我们使用慈鲷PPI来研究口孵育的转录组特征，口孵育是一个伴随自愿饥饿的父母照顾的急性期[3]。来自口孵的博氏Astatotilapia burtoni的脑转录组数据用于对所得的相互作用组进行加权。该项目提出了一种方法来表征转录组的变化，在非模式生物利用模式生物的资源。
Protein-protein interactomes (PPIs) have been used extensively to classify and predict genes associated with complex phenotypes. PPI approaches have been successful for organisms with well-annotated genome features, but are more challenging for non-model organisms due to a lack of available experimental data. To address this challenge, labs have created PPIs for non-model organisms by inferring" interologs", or orthologous protein-protein interactions [1]. African cichlids are a diverse clade of fishes that have been used to study rapid evolutionary divergence [3]. This project presents an interolog-based method of building an African cichlid-specific PPI from an existing zebrafish interactome [1]. As a case study, we use a cichlid PPI to study the transcriptomic signatures of mouthbrooding, an acute period of parental care accompanied by voluntary starvation [3]. Brain transcriptome data from mouthbrooding Astatotilapia burtoni were used to weight the resulting interactome. This project presents a method to characterize transcriptomic changes in a non-model organism using resources from model organisms.
最近收集的丽鱼种群中的产妇护理和社会表型的改变。
影响因子：
2.6
作者：
S. Renn;Julia B. Carleton;H. Magee;M. L. Nguyen;A. W. Tanner
通讯作者：
A. W. Tanner