A NOVEL TYPE OF B-CELL MITOGEN ISOLATED FROM JUZEN-TAIHO-TO (TJ-48), A JAPANESE TRADITIONAL MEDICINE
A NOVEL TYPE OF B-CELL MITOGEN ISOLATED FROM JUZEN-TAIHO-TO (TJ-48), A JAPANESE TRADITIONAL MEDICINE
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DOI：
10.1016/0192-0561(94)00056-5
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发表时间：
1994-11-01
期刊：
影响因子：
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通讯作者：
KAWAMURA, H
中科院分区：
文献类型：
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作者：
TAKEMOTO, N;KIYOHARA, H;KAWAMURA, H
据悉，日本传统中药“橘子参太和藤”（TJ-48）具有诱导b细胞增殖等多种免疫活性。我们研究了对脾细胞增殖最活跃的TJ-48 （F-5-2）果胶多糖部分的性质。F-5-2在与TJ-48相同的程度上诱导了b细胞的增殖，尤其是同时含有sIgM和sIgD的b细胞。F-5-2诱导的增殖不依赖t细胞，依赖巨噬细胞。巨噬细胞可以替代巨噬细胞分泌的可溶性因子，但不能替代IL-1。一般来说，已知b细胞有丝分裂原诱导b细胞增殖并随后分化为浆细胞。然而，F-5-2虽然能诱导b细胞分化，但在b细胞分化的中间阶段阻滞了b细胞的发育。F-5-2诱导的b细胞对IL-6和抗原（SRBC）产生IgM抗体，但不产生IgG抗体。F-5-2诱导IL-6R不仅在IgM（+）和IgG(+) b细胞上表达，而且在IgD(+) b细胞上表达。这些结果提示F-5-2是一种新型的b细胞有丝分裂原。
Juzen-taiho-to (TJ-48), a Japanese traditional medicine, is known to have various immunological activities including the induction of B-cell proliferation. We investigated the properties of the pectic polysaccharide fraction of TJ-48 (F-5-2) which is most active in the proliferation of spleen cells. To an extent equal to that of TJ-48, F-5-2 induced the proliferation of B-cells, particularly those holding both sIgM and sIgD. The proliferation induced by F-5-2 was T-cell independent and macrophage dependent. The macrophages could be substituted for a soluble factor(s) secreted from the macrophages but not for IL-1. Generally, B-cell mitogens are known to induce the proliferation of B-cells and subsequently differentiation into plasma cells. However,although F-5-2 induced the B-cell differentiation, it arrested their development in the intermediate stage of the B-cell differentiation. The B-cells induced by F-5-2 produced IgM antibody in response to IL-6 and an antigen (SRBC) but not IgG antibody. F-5-2 induced the expression of IL-6R not only on IgM(+) and IgG(+) B-cells but also on IgD(+) B-cells. These results suggest that F-5-2 is a new type of B-cell mitogen.