Effects of Buthus martensii Karsch Venom on Cell Proliferation and Cytotoxicity in Hela Cells
Effects of Buthus martensii Karsch Venom on Cell Proliferation and Cytotoxicity in Hela Cells
复制标题
DOI：
10.4028/www.scientific.net/amr.345.393
复制
发表时间：
2011-09
期刊：
影响因子：
--
通讯作者：
Zhi Wang;W. Fu;Xianghuai Lu;Guang-xian Cai
中科院分区：
文献类型：
--
作者：
Zhi Wang;W. Fu;Xianghuai Lu;Guang-xian Cai
本文主要研究马氏蝎毒对人宫颈癌Hela细胞株增殖的影响及其分子机制。MTT实验显示，蝎子毒液对Hela细胞的增殖具有时间依赖性和浓度依赖性，50%抑制浓度（IC50）为34.5 μg/mL（48 h）。流式细胞术检测发现蝎毒可诱导Hela细胞凋亡和坏死。RT-PCR和Western blot结果显示，蝎子毒液对Hela细胞p21蛋白的表达有明显上调，而p21 mRNA的表达无显著差异。这些结果提示，蝎子毒液的作用机制可能是激活p21蛋白的表达，导致Hela细胞凋亡。
This paper focuses on the effect of the venom of the scorpion Buthus martensii on the proliferation of human cervical carcinoma Hela cell line and the related molecular mechanism. MTT test showed that the scorpion venom inhibited proliferation of Hela cells in time-dependent and concentration-dependent manner with 50% inhibitory concentration (IC50) of 34.5 μg/mL(48 h). By using flow cytometry, it was found that the scorpion venom could induce apoptosis and necrosis in Hela cells. RT-PCR and Western blot indicated there were obviously up-regulated in the expressions of p21 protein but the expression of p21 mRNA showed no significant difference in the Hela cell by the scorpion venom. These results suggest that the possible mechanism of the scorpion venom is to activate the expressions of p21 protein and to cause Hela cell apoptosis.