Specific representation of cloned repetitive DNA sequences in sea urchin RNAs
Specific representation of cloned repetitive DNA sequences in sea urchin RNAs
复制标题
海胆 RNA 中克隆的重复 DNA 序列的具体表示
DOI：
10.1016/0092-8674(78)90094-6
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发表时间：
1978
期刊：
影响因子：
64.5
通讯作者：
E. Davidson
中科院分区：
文献类型：
--
作者：
R. Scheller;F. Costantini;M. Kozlowski;R. Britten;E. Davidson
九个克隆的重复序列被标记、链分离并分别与从原肠胚阶段海胆胚胎和成年海胆肠细胞的细胞核中提取的RNA杂交。通过RNA过量杂交动力学和DNA过量滴定法测量与每个克隆序列互补的转录物浓度。每个 RNA 中某些重复家族的转录物浓度是其他家族转录物浓度的 100 倍以上。肠核 RNA 中高度代表的重复序列家族集与原肠胚核 RNA 中高度代表的重复序列家族不同。与用成熟卵母细胞 RNA 获得的结果以及 Costantini 等人 (1978) 在随附论文中提出的结果一起，这些发现表明 RNA 中重复序列表示的定量模式对于每种细胞类型是特定的。所有九个克隆重复序列的两条链都在某种程度上代表了所有研究的 RNA。通常，尽管并非总是如此，与每个重复的两条链互补的转录物浓度的差异不会超过两倍。克隆的示踪剂不与多聚体信使RNA发生反应，并且与其杂交的核RNA分子比重复序列本身大许多倍。
Nine cloned repetitive sequences were labeled, strand-separated and individually hybridized with RNA extracted from the nuclei of gastrula stage sea urchin embryos and of adult sea urchin intestine cells. The concentration of transcripts complementary to each cloned sequence was measured by RNA excess hybridization kinetics and by a DNA excess titration method. Transcripts of certain of the repeat families are present at over 100 times the concentration of transcripts of other families in each RNA. The set of repetitive sequence families highly represented in intestine nuclear RNA is different from that highly represented in gastrula nuclear RNA. Together with the results obtained with mature oocyte RNA and presented in the accompanying paper by Costantini et al.(1978), these findings show that quantitative patterns of repetitive sequence representation in RNA are specific to each cell type. Both strands of all of the nine cloned repeats are represented at some level in all the RNAs studied. Usually, though not always, the concentrations of transcripts complementary to the two strands of each repeat do not differ by more than a factor of two. The cloned tracers do not react with polysomal messenger RNA, and the nuclear RNA molecules with which they hybridize are many times larger than the repetitive sequences themselves.