Expression of innate immune genes, proteins and microRNAs in lung tissue of pigs infected experimentally with influenza virus (H1N2)
Expression of innate immune genes, proteins and microRNAs in lung tissue of pigs infected experimentally with influenza virus (H1N2)
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DOI：
10.1177/1753425912473668
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发表时间：
2013-10-01
期刊：
影响因子：
3.2
通讯作者：
Heegaard, Peter M. H.
中科院分区：
文献类型：
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作者：
Skovgaard, Kerstin;Cirera, Susanna;Heegaard, Peter M. H.
本研究旨在更好地了解包括miRNA在内的先天性免疫因子参与流感病毒感染的局部宿主反应。用甲型H1 N2亚型流感病毒攻击20头猪。在攻击后不同时间点（感染后24小时、72小时和14天（p.i.））定量肺组织中微小RNA（miRNA）、mRNA和蛋白质的表达。包括模式识别受体在内的几组基因在不同的时间点和感染状态下受到显著的调控（TLR 2、TLR 3、TLR 7、视黄酸诱导基因I、黑素瘤分化相关蛋白-5）、IFN和IFN诱导基因（IFN-、IFN-、IRF 7、STAT 1、ISG 15和OASL）、细胞因子（IL-1、IL-1 RN、IL-6、IL-7、IL-10、IL-12 A、TNF-、CCL 2、CCL 3和CXCL 10）和几种急性期蛋白。同样，与对照猪相比，以下miRNA在一个或多个时间组中差异表达：miR-15 a、miR-21、miR-146、miR-206、miR-223和miR-451。在d1 p.i.肺组织IL-6、IL-12和IFN-γ蛋白水平在感染后第3天显著高于对照组，结合珠蛋白和C-反应蛋白水平在感染后第3天显著高于对照组。我们的研究结果表明，除了广泛的先天免疫因素外，miRNA也可能参与控制猪的急性流感感染。
This study aimed at providing a better understanding of the involvement of innate immune factors, including miRNA, in the local host response to influenza virus infection. Twenty pigs were challenged by influenza A virus subtype H1N2. Expression of microRNA (miRNA), mRNA and proteins were quantified in lung tissue at different time points after challenge (24h, 72h and 14d post-infection (p.i.). Several groups of genes were significantly regulated according to time point and infection status including pattern recognition receptors (TLR2, TLR3, TLR7, retinoic acid-inducible gene I, melanoma differentiation associated protein-5), IFN and IFN-induced genes (IFN-, IFN-, IRF7, STAT1, ISG15 and OASL), cytokines (IL-1 , IL-1RN, IL-6, IL-7, IL-10, IL-12A, TNF-, CCL2, CCL3 and CXCL10) and several acute phase proteins. Likewise, the following miRNAs were differentially expressed in one or more time groups compared with the control pigs: miR-15a, miR-21, miR-146, miR-206, miR-223 and miR-451. At d 1 p.i. lung tissue protein levels of IL-6, IL-12 and IFN- were significantly increased compared with the control group, and haptoglobin and C-reactive protein were significantly increased at d 3 p.i. Our results suggest that, in addition to a wide range of innate immune factors, miRNAs may also be involved in controlling acute influenza infection in pigs.