DETECTION AND TYPING OF FOOT-AND-MOUTH-DISEASE VIRUS BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY - A SENSITIVE, RAPID AND RELIABLE TECHNIQUE FOR PRIMARY DIAGNOSIS
DETECTION AND TYPING OF FOOT-AND-MOUTH-DISEASE VIRUS BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY - A SENSITIVE, RAPID AND RELIABLE TECHNIQUE FOR PRIMARY DIAGNOSIS
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DOI：
10.1016/s0034-5288(18)30778-1
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发表时间：
1987-09-01
影响因子：
2.4
通讯作者：
SMITH, PML
中科院分区：
文献类型：
--
作者：
ROEDER, PL;SMITH, PML
本文描述了一种高灵敏度的间接夹心酶联免疫吸附试验，适用于所有七种血清型口蹄疫病毒的常规诊断和分型试验。该检测使用针对灭活146 S病毒抗原的兔和豚鼠抗血清。与全病毒体146 S和衍生病毒体亚单位12 S抗原的强同型和最小异型反应达到补体结合试验的约125倍的检测灵敏度。当应用于诊断材料与阳性-阴性阈值标准产生的测试大量的阴性样本，获得了阳性结果的83.3%的原始病毒阳性上皮细胞，三倍的补体结合试验的速度，并在一次传代培养后，所有的类型。
A highly sensitive indirect sandwich enzyme-linked immunosorbent assay suitable for adoption as the routine diagnostic and typing test for foot-and-mouth disease virus of all seven serotypes is described. The assay uses rabbit and guinea-pig antisera raised against inactivated 146S virus antigens. Strong homotypic and minimal heterotypic reactions with both whole virion 146S and derived virion subunit 12S antigens achieved a detection sensitivity approximately 125 times that of the complement fixation test. When applied to diagnostic material with positive-negative threshold criteria generated from testing a large number of negative samples, a positive result was obtained on 83.3 per cent of original virus-positive epithelia, three times the rate for the complement fixation test, and all were typed after one passage in culture.