[Pathological changes in exposed pulp tissues in germ-free rats].
[Pathological changes in exposed pulp tissues in germ-free rats].
复制标题
无菌大鼠裸露牙髓组织的病理变化[J].
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
H. Yoshida
中科院分区：
文献类型：
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作者：
H. Yoshida
本研究的目的是观察无菌Wistar大鼠暴露牙髓的病理变化，并与常规大鼠比较。用19号刚玉尖或1/2号圆钻暴露大鼠牙髓组织，使暴露不封闭。为了研究新生钙化组织的生长情况，每隔5天给大鼠腹腔注射乙二胺四乙酸铅二钠水溶液。分别于牙髓暴露后14天和28天处死动物。结果如下：1.在常规大鼠牙髓组织中，可见较高比例的广泛坏死和炎性细胞浸润，甚至可见根尖周脓肿。未见牙本质桥形成。2.在无菌组大鼠牙髓组织中，虽然观察到成牙本质细胞的紊乱、成纤维细胞的增殖和炎症细胞的轻度浸润，但暴露后14天有30%的牙本质桥形成，28天有57%的牙本质桥形成。然而，在牙本质桥中，通过时间标记方法得到的导线并不清楚。3.无论是常规组还是无菌组大鼠牙髓组织的损伤程度均明显高于碳化硅尖组。4.8~11周龄无菌大鼠牙髓损伤组织学改变与29周龄无菌大鼠牙髓组织学改变无明显差异。
The purpose of this study was to observe the pathological changes in the exposed pulp in the germ-free Wistar rats compared with those in the conventional rats. Pulp tissues of the rats were exposed with No. 19 carborundum point or No. 1/2 round bur and the exposures were left unsealed. In order to study the growth of the newly produced calcified tissues, the rats were injected intraperitoneally every 5 days with an aqueous solution of lead disodium ethylene diamine tetraacetate. The animals were sacrificed at 14 and 28 days after the pulp exposure. The results obtained were as follows: 1. In the pulp tissues of the conventional rats, a high percentage of extensive necrosis and inflammatory cell infiltration was observed and even periapical abscess was recognized frequently. Dentine bridge formation was not seen. 2. In the pulp tissues of the germ-free rats, although odontoblast disturbance, proliferation of fibroblasts and slight infiltration of the inflammatory cells were observed, dentine bridge formation occurred in 30 per cent of the teeth in 14 days and 57 per cent in 28 days after the pulp exposure. The lead lines by the time-marking method, however, were not clearly seen in the dentine bridge. 3. Pulp tissues exposed with a round bur were more damaged than those exposed with a carborundum point in both the conventional and germ-free rats. 4. There was no apparent difference between the histologic changes of the injured pulp tissues in the 8 to 11-week-old germ-free rats and those in the 29-week-old rats.