Double isotype production by a neoplastic B cell line. II. Allelically excluded production of mu and gamma 1 heavy chains without CH gene rearrangement.
Double isotype production by a neoplastic B cell line. II. Allelically excluded production of mu and gamma 1 heavy chains without CH gene rearrangement.
复制标题
肿瘤 B 细胞系产生双同种型。二.等位基因排除 mu 和 gamma 1 重链的产生，无需 CH 基因重排。
DOI：
10.1084/jem.164.2.562
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发表时间：
1986-08-01
影响因子：
15.3
通讯作者：
Tucker, P W
中科院分区：
文献类型：
--
作者：
Chen, Y W;Word, C;Dev, V;Uhr, J W;Vitetta, E S;Tucker, P W
在我们的随附文件中，我们描述了一个开关变异体(BCL1.2.58)，它表达膜和分泌型的IgM和IgG1。IgM和IgG1具有相同的独特型，并使用相同的VDJ重排。在这里，对亲本(BCL1.B1)和变异体(BCL1.B2)DNA的Ig重链(Ig CH)整个恒定区的详细Southern杂交分析表明没有检测到重排。对JH-C Mu区的类似分析得出结论，产生IgM/IgG1的变异体中的两个重链等位基因携带相同的VDJ重排，但其3‘侧翼区不同。一条12号染色体不携带任何Ig CH基因，而另一条12号染色体携带一份CH基因拷贝。然而，在BCL1.B1中，12号染色体上的每个等位基因都携带一个完整的CH基因副本。核型分析证实，在BCL1.2.58和BCL1.B1细胞中都存在两条t(12；16)易位染色体，在12号染色体远端(12F2)和16号染色体着丝粒(16B3)下方的VH基因座上有一个5‘的断裂。我们得出结论：BCL1.B2中IgM和IgG1的双重产生是通过在同一条染色体上使用单个VDJ以种系构型转录相应的CH基因来完成的。
In our accompanying paper, we described a switch variant (BCL1.2.58) that expresses membrane and secreted forms of IgM and IgG1. Both IgM and IgG1 share the same idiotype and use the same VDJ rearrangement. Here, a detailed Southern blot analysis of the entire constant region of the Ig heavy chain (Ig CH) locus of parental (BCL1.B1) and variants (BCL1.B2) DNA showed no detectable rearrangement. Similar analysis of the JH-C mu region led to the conclusion that two heavy chain alleles present in the IgM/IgG1-producing variants carried the same VDJ rearrangement but differed in their 3' flanking regions. One chromosome 12 did not carry any Ig CH genes, whereas, the other chromosome 12 carried one copy of CH genes. In BCL1.B1, however, each of the chromosome 12 alleles carried a full copy of CH genes. Karyotypic analysis confirmed the presence of two translocated t(12;16) chromosomes in both BCL1.2.58 and BCL1.B1 cells, with a break 5' to the VH locus at the distal region (12F2) of chromosome 12, and at the proximal region below the centromere (16B3) of chromosome 16. We conclude that double production of IgM and IgG1 in BCL1.B2 is accomplished by transcription of the corresponding CH genes in germline configuration using a single VDJ on the same chromosome 12.