Continuous evolution of influenza A viruses of swine from 2013 to 2015 in Guangdong, China
Continuous evolution of influenza A viruses of swine from 2013 to 2015 in Guangdong, China
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DOI：
10.1371/journal.pone.0217607
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发表时间：
2019-07-19
期刊：
影响因子：
3.7
通讯作者：
Zhang, Guihong
中科院分区：
文献类型：
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作者：
Cao, Zhenpeng;Zeng, Weijie;Zhang, Guihong
中国南方被认为是流感病毒大流行的重要来源，因为在家禽和猪中有大量不同的病毒宿主。为了研究猪A型流感病毒（IAV-S）的趋势，2013 - 2015年在中国广东省开展了一项主动监测计划。系统发育分析表明，分离株的外部基因通过多次替换被分配到欧亚类鸟猪（EA） H1N1和/或类人H3N2谱系，表明遗传转移显著。此外，不同来源的内部基因（PB2、PB1、PA、NP: pdm/09（2009大流行性流感病毒）-起源、M: pdm/09-或ea -起源、NS：北美三重重组(TR)-起源）已成为华南地区IAV-S的主要骨干。根据8个基因片段的来源，分离物可分为5个基因型。小鼠实验结果显示，YJ4（基因1型）和DG2（基因4型）对小鼠致病性最强，在肾脏和大脑中观察到病毒，提示全身性感染。IAV-S基因组成的改变支持继续实施对IAV-S的强化监测，并更加关注向人类传播的潜在转变。
Southern China is considered an important source of influenza virus pandemics because of the large, diverse viral reservoirs in poultry and swine. To examine the trend in influenza A virus of swine (IAV-S), an active surveillance program has been conducted from 2013 to 2015 in Guangdong, China. The phylogenetic analyses showed that the external genes of the isolates were assigned to the Eurasian avian-like swine (EA) H1N1 and/or human-like H3N2 lineages with multiple substitutions, indicating a notable genetic shift. Moreover, the internal genes derived from different origins (PB2, PB1, PA, NP: pdm/09 (pandemic influenza virus 2009)-origin, M: pdm/09- or EA-origin, NS: North American Triple Reassortant (TR)-origin have become the dominant backbone of IAV-S in southern China. According to the origins of the eight gene segments, the isolates can be categorized into five genotypes. The results of mice experiment showed that the YJ4 (genotype 1) and DG2 (genotype 4) are the most pathogenic to mice, and the viruses are observed in kidneys and brains, indicating the systemic infection. The alterations of the IAV-S gene composition supported the continued implementation of the intensive surveillance of IAV-S and the greater attention focused on potential shifts toward transmission to humans.