Matrix metalloproteinases.
Matrix metalloproteinases.
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DOI：
10.1002/0471143030.cb1008s40
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发表时间：
2008-09-01
影响因子：
--
通讯作者：
Birkedal-Hansen, Bente
中科院分区：
文献类型：
--
作者：
Birkedal-Hansen, Henning;Yamada, Susan;Birkedal-Hansen, Bente
基质金属蛋白酶是一类在细胞外基质的重塑和肿瘤转移中起重要作用的酶。本单元描述了一套方法--细胞介导的1型胶原纤维溶解，直接和反向酶谱，基于α 2-巨球蛋白和TIMP-1和-2的酶捕获，以及金属蛋白酶前体中隐藏的巯基的证明--这些方法用于表征基质金属蛋白酶及其抑制剂的功能。
Matrix metalloproteinases are a class of enzymes that play an important role in the remodeling of the extracellular matrix in development and cancer metastasis. This unit describes a set of methods--cell-mediated dissolution of type-1 collagen fibrils, direct and reverse zymography, enzyme capture based on alpha2-macroglobulin and TIMP-1 and -2, and demonstration of cryptic thiol groups in metalloproteinase precursors--that are used to characterize the functions of matrix metalloproteinases and their inhibitors.