Development of an attenuated live vaccine candidate of duck Tembusu virus strain
Development of an attenuated live vaccine candidate of duck Tembusu virus strain
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鸭天布苏病毒株候选减毒活疫苗的研制
DOI：
10.1016/j.vetmic.2019.03.022
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发表时间：
2019-04-01
影响因子：
3.3
通讯作者：
Diao, Youxiang
中科院分区：
文献类型：
--
作者：
He, Dalin;Zhang, Xin;Diao, Youxiang
鸭坦布苏病毒（DTMUV）感染可给中国养鸭业造成巨大的经济损失。在这项研究中，我们从鸭场附近的麻雀中分离出一株DTMUV （SDS）毒株，并通过在特定的无病原体的鸡和鸭胚胎中连续传代（交替100代）来减弱它。我们在第60代（SDS-60）后减毒，基于产生的胚胎没有可见的病变并且仍然存活。选择病毒滴度为10(-2.46)EID50的第70株（SDS-70）作为减毒活疫苗。用SDS-70毒株免疫雏鸭后，雏鸭对SDS-10毒株的感染具有100%的保护作用。我们的数据表明，疫苗可以保护鸭子免受TMUV感染。我们的研究还表明，这种新开发的减毒候选疫苗具有良好的免疫原性，是安全的，并且具有控制鸭DTMUV感染的潜力。
Infection with duck Tembusu virus (DTMUV) can cause large economic losses to the duck-rearing industry in China. In this study, we isolated a virulent strain of DTMUV (SDS) from sparrows near a duck farm and attenuated it via serially passaging (alternately for 100 passages) in specific pathogen-free chicken and duck embryos. We attenuated the virus after the 60th passage (SDS-60), based on the production of embryos that were free of visible lesions and still alive. The 70th adapted strain (SDS-70), obtained with a virus titer of 10(-2.46) EID50 was chosen to be the live attenuated vaccine. After immunizing ducklings with the SDS-70 strain, they obtained 100% protection against infection by the SDS-10 virulent strain. Our data demonstrate that the vaccine can protect ducks from becoming infected with TMUV. Our study also shows that this newly developed attenuated vaccine candidate provides excellent immunogenicity, is safe, and has the potential to control DTMUV infections in ducks.