Electrochemical genosensors for the detection of cancer-related miRNAs
Electrochemical genosensors for the detection of cancer-related miRNAs
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DOI：
10.1007/s00216-013-7459-z
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发表时间：
2014-01-01
影响因子：
4.3
通讯作者：
Pingarron, Jose M.
中科院分区：
文献类型：
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作者：
Campuzano, Susana;Pedrero, Maria;Pingarron, Jose M.
MicroRNA（miRNAs）是人类遗传学的新星，是一类天然存在的19-25个碱基的非编码RNA，在转录后调节基因表达，是肿瘤诊断和预后的良好生物标志物。由于它们的长度短、序列相似性和非常低的浓度，它们在真实的样品中的检测具有挑战性。在用于miRNA检测的其他方法中，电化学核酸生物传感器在高灵敏度、易用性、短测定时间、无毒实验步骤和对即时检测的适应性方面表现出相关优势。本文就近年来快速发展的用于miRNA检测的电化学生物传感器的研究进展作一综述。不同的策略，以实现新的信号放大和灵敏的电化学检测的基本原理进行了讨论，并突出了相关方法的一些例子，沿着未来的前景和挑战。
MicroRNAs (miRNAs), new stars of human genetics, are naturally occurring, 19-25 base pair, noncoding RNAs that regulate gene expression posttranscriptionally and have been demonstrated to be excellent biomarkers for cancer diagnosis/prognosis. Because of their short length, sequence similarity, and very low concentration, their detection in real samples is challenging. Among other methods for miRNA detection, electrochemical nucleic acid biosensors exhibit relevant advantages in terms of high sensitivity, ease of use, short assay time, nontoxic experimental steps, and adaptability to point-of-care testing. This article gives a brief overview of recent advances in the rapidly developing area of electrochemical biosensors for miRNA detection. The fundamentals of the different strategies developed to achieve novel signal amplification and sensitive electrochemical detection are discussed, and some examples of relevant approaches are highlighted, along with future prospects and challenges.