Differential expression of moesin in cells of hematopoietic lineage and lymphatic systems
Differential expression of moesin in cells of hematopoietic lineage and lymphatic systems
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DOI：
10.1007/s004180050262
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发表时间：
1998-07-01
影响因子：
2.3
通讯作者：
Taniguchi, S
中科院分区：
文献类型：
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作者：
Masumoto, J;Sagara, J;Taniguchi, S
Moesin是ERM家族的成员，由Ezrin、Radioxin和Moesin组成。该蛋白位于质膜上，类似于Ezrin和Radixin，被认为调节细胞运动和形态变化。用免疫沉淀法和免疫印迹法证实其特异性的单抗CR22，对福尔马林固定和石蜡包埋的各种人体组织，特别是骨髓凝块和淋巴组织进行免疫组织化学染色，以检测造血系和淋巴系统细胞中moesin的表达。在骨髓中，Moesin在髓系细胞中表达，而在红系细胞中几乎不表达。Moesin在成熟巨核细胞的中心和外围均有高表达。淋巴组织中，副皮质中的T淋巴细胞表达Moesin。在末梢中心周围的外套带，Moesin由小淋巴细胞表达，经鉴定为B淋巴细胞。此外，在炎症区域，Moesin在中性粒细胞的中心和外围均有表达，而在远处的一些中性粒细胞中，Moesin定位于细胞外围。这些结果表明，Moesin在这些细胞中的差异表达与它们的形态和特殊功能有关。
Moesin is a member of the ERM family consisting of ezrin, radixin, and moesin. The protein is located in the plasma membrane similarly to ezrin and radixin, and is thought to regulate cellular movements and morphological changes. Using monoclonal antibody CR22, the specificity of which against human moesin was confirmed by immunoprecipitation and western blotting analysis, we immunohistochemically stained various formalin-fixed and paraffin-embedded human tissues, in particular, clots of bone marrow and lymphatic tissues, to examine moesin expression in cells of hematopoietic lineage and lymphatic systems. In the bone marrow, moesin was expressed in myeloid cells, while little staining was detected in erythroid cells. Moesin was highly expressed in both the center and the periphery of mature megakaryocytes. In the lymphatic tissues, moesin was strongly expressed by T-lymphocytes in the paracortex. In the mantle zone, the periphery of the terminal center, moesin was expressed by small lymphocytes which were identified as B-lymphocytes. Furthermore, in areas of inflammation, moesin was expressed in both the center and the periphery of neutrophils, whereas in some neutrophils in distant areas, moesin was localized at the cellular periphery. These results suggest that differential expression of moesin in these cells is involved in their morphology and specialized functions.