Class I histone deacetylases in retinal progenitors and differentiating ganglion cells.
Class I histone deacetylases in retinal progenitors and differentiating ganglion cells.
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DOI：
10.1016/j.gep.2018.08.007
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发表时间：
2018-12
期刊：
影响因子：
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通讯作者：
Ankita Saha;Sarika Tiwari;S. Dharmarajan;D. Otteson;T. Belecky-Adams
中科院分区：
文献类型：
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作者：
Ankita Saha;Sarika Tiwari;S. Dharmarajan;D. Otteson;T. Belecky-Adams
背景组蛋白的乙酰化状态已被用作祖细胞和分化细胞发育状态的指标。本研究的目的是确定I类组蛋白去乙酰化酶（HDAC）在视网膜祖细胞（RPC）和视网膜神经节细胞（RGCs）的核定位模式，作为了解其潜在的重要性在鼠retina.Results细胞命运的第一步，唯一的HDAC标记RPC核在E16和P5是HDAC1。相反，在分化的RGC中，所有I类HDAC的核定位普遍增加。在P5和P30之间，SOX 2的表达局限于Müller神经胶质细胞、胆碱能无长突细胞和视网膜星形胶质细胞。胆碱能无长突蛋白显示I类HDAC核定位变化的组合。值得注意的是，尽管Müller胶质细胞和视网膜星形胶质细胞表达许多相同的基因，但P30 Müller胶质细胞仅表达HDAC1，而视网膜星形胶质细胞表达HDAC1、2和3。结论一种或多种I类HDACs可能在视网膜细胞类型特异性分化中起作用。
Background The acetylation state of histones has been used as an indicator of the developmental state of progenitor and differentiating cells. The goal of this study was to determine the nuclear localization patterns of Class I histone deacetylases (HDACs) in retinal progenitor cells (RPCs) and retinal ganglion cells (RGCs), as the first step in understanding their potential importance in cell fate determination within the murine retina.Results The only HDAC to label RPC nuclei at E16 and P5 was HDAC1. In contrast, there was generally increased nuclear localization of all Class I HDACs in differentiating RGCs. Between P5 and P30, SOX2 expression becomes restricted to Müller glial, cholinergic amacrine cells, and retinal astrocytes. Cholinergic amacrine showed a combination of changes in nuclear localization of Class I HDACs. Strikingly, although Müller glia and retinal astrocytes express many of the same genes, P30 Müller glial cells showed nuclear localization only of HDAC1, while retinal astrocytes were positive for HDACs 1, 2, and 3.Conclusion These results indicate there may be a role for one or more of the Class I HDACs in retinal cell type-specific differentiation.