Effects of ovariectomy and estrogen replacement therapy on arthritis and bone mineral density in rats with collagen-induced arthritis
Effects of ovariectomy and estrogen replacement therapy on arthritis and bone mineral density in rats with collagen-induced arthritis
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DOI：
10.1016/s8756-3282(01)00426-4
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发表时间：
2001-06-01
期刊：
影响因子：
4.1
通讯作者：
Teshima, R
中科院分区：
文献类型：
--
作者：
Yamasaki, D;Enokida, M;Teshima, R
我们研究了卵巢切除术（ovx）和雌激素替代治疗（ERT）对胶原诱导性关节炎（CIA）大鼠骨密度（BMD）和关节炎严重程度的影响。将7月龄雌性SD大鼠分为假手术组（n = 8）、CIA组（n = 24）、ovx组（n = 10）、CIA + ovx组（n = II）和CIA + ovx + ERT组（n = 14）。在这些组中，在致敏后7天进行ovx，并在致敏后8天开始ERT（17 β-雌二醇，20 μ g/kg，每周三次）。每2周，直到致敏后8周，评价关节炎评分和后爪厚度，并通过外周定量计算机断层扫描测量胫骨干骺端和骨干中的骨小梁和皮质骨的BMD。在致敏后的所有时间点，CIA + ovx组的关节炎评分最高。在致敏后8周，CIA + ovx组的后爪厚度显著高于CIA组（p < 0.05）。CIA + ovx + ERT组的关节炎评分和后爪厚度均低于CIA + ovx组。致敏后4、6和8周，CIA + ovxr组的松质骨和皮质骨干骺端BMD均显著低于CIA组。在CIA + ovx组中，与0周时相比，8周时干骺端骨小梁BMD变化-34 +/-11%，皮质BMD变化-14 +/-7%。在CIA组中，干骺端骨密度的变化在松质骨中为-7 +/- 11%，在皮质骨中为0 +/- 7%。干骺端松质骨和皮质骨丢失差异有显著性（均P < 0.01）。致敏后6周和8周，CIA + ovx + ERT组的BMD降低显著低于CIA + ovx组。尽管CW组骨干BMD也降低，但降低程度小于干骺端。我们的结论是，ovx在CIA大鼠可以增加关节炎和骨丢失的严重程度，和ERT可以抑制关节炎和骨丢失。(C)2001年，Elsevier Science Inc. All rights reserved.
We investigated the effects of ovariectomy (ovx) and estrogen replacement therapy (ERT) on bone mineral density (BMD) and arthritis severity in rats with collagen-induced arthritis (CIA). Seven-month-old female Sprague-Dawley rats were separated into a sham group (n = 8), CIA group (n = 24), ovx group (n = 10), CIA + ovx group (n = II), and CIA + ovx + ERT group (n = 14). In these groups, ovx was performed at 7 days, and ERT (17 beta -estradiol at 20 mug/kg three times per week) was initiated 8 days after sensitization. Every 2 weeks, until 8 weeks after sensitization, arthritis score and hind paw thickness were evaluated, and BMD of the trabecular and cortical hones in the metaphysis and diaphysis of the tibia were measured by peripheral quantitative computed tomography, The arthritis score was highest in the CIA + ovx group at all timepoints after sensitization. The hind paw thickness was significantly higher in the CIA + ovx group than in the CIA group at 8 weeks after sensitization (p < 0.05). Both the arthritis score and hind paw thickness were lower in the CIA + ovx + ERT group than in the CIA + ovx group. BMD in the metaphysis was significantly decreased in both the trabecular and cortical bones in the CIA + ovxr group compared with those in the CIA group at 4, 6, and 8 weeks after sensitization. In the CIA + ovx group, trabecular BMD was changed by -34 +/- 11%, and cortical BMD changed by -14 +/- 7% in the metaphysis at 8 weeks compared with those at 0 week. In the CIA group, changes of BmD in the metaphyis were -7 +/- 11% in trabecular bone and 0 +/- 7% in cortical bone. These differences of trabecular and cortical bone loss in the metaphysis were significant (both p < 0.01). BMD reduction vas significantly less in the CIA + ovx + ERT group than in the CIA + ovx group at 6 and 8 weeks after sensitization. Although BMD in the diaphysis was also reduced in the groups crith CW, the degree of reduction was smaller than in the metaphysis. We conclude that ovx in CIA rats could enhance the severity of arthritis and bone loss, and that ERT could suppress arthritis and bone loss. (C) 2001 by Elsevier Science Inc. All rights reserved.