Integrated Proteomic and Transcriptomic Analyses Reveal the Roles of Brucella Homolog of BAX Inhibitor 1 in Cell Division and Membrane Homeostasis of Brucella suis S2.
Integrated Proteomic and Transcriptomic Analyses Reveal the Roles of Brucella Homolog of BAX Inhibitor 1 in Cell Division and Membrane Homeostasis of Brucella suis S2.
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综合蛋白质组学和转录组学分析揭示了 BAX 抑制剂 1 的布鲁氏菌同源物在猪布鲁氏菌 S2 细胞分裂和膜稳态中的作用
DOI：
10.3389/fmicb.2021.632095
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发表时间：
2021
影响因子：
5.2
通讯作者：
Wang A
中科院分区：
文献类型：
--
作者：
Zhang G;Zhong F;Chen L;Qin P;Li J;Zhi F;Tian L;Zhou D;Lin P;Chen H;Tang K;Liu W;Jin Y;Wang A
BAX抑制剂1（BI-1）是一种进化上保守的跨膜蛋白，在筛选抑制酵母细胞中BAX诱导的细胞凋亡的人蛋白质的过程中首次鉴定。真核生物BI-1是一种细胞保护蛋白，可抑制真核生物中由多种刺激诱导的细胞死亡。布鲁氏菌是危害人类健康和畜牧业的布鲁氏菌病的病原体，其基因保守，编码BI-1样蛋白。为探讨布鲁氏菌BI-1同源物BrBI在猪布鲁氏菌S2中的作用，构建了brbI缺失突变株及其互补株。brbI缺失改变了猪种布氏杆菌S2的膜特性，并降低了其对酸性pH、H2 O2、多粘菌素B和林可霉素的耐受性。此外，删除brbI导致猪布鲁氏菌S2的生长、细胞分裂和活力缺陷。然后，我们通过整合转录组学和蛋白质组学分析揭示了brbI缺失对猪布鲁氏菌S2生理特性的影响。综合分析表明，brbI缺失在mRNA和/或蛋白水平上显著影响多个基因的表达。具体而言，受影响的分裂体蛋白FtsB、FtsI、FtsL和FtsQ可能是brbI突变株细胞分裂受损的分子基础，而广泛受影响的膜蛋白和转运蛋白相关蛋白与brbI突变株细胞膜特性改变的表型一致。总之，我们的研究结果表明，BrBI是一种细菌的细胞保护蛋白，参与膜稳态，细胞分裂，并在猪布鲁氏菌S2的应激抗性。
BAX inhibitor 1 (BI-1) is an evolutionarily conserved transmembrane protein first identified in a screening process for human proteins that suppress BAX-induced apoptosis in yeast cells. Eukaryotic BI-1 is a cytoprotective protein that suppresses cell death induced by multiple stimuli in eukaryotes. Brucella, the causative agent of brucellosis that threatens public health and animal husbandry, contains a conserved gene that encodes BI-1-like protein. To explore the role of the Brucella homolog of BI-1, BrBI, in Brucella suis S2, we constructed the brbI deletion mutant strain and its complemented strain. brbI deletion altered the membrane properties of Brucella suis S2 and decreased its resistance to acidic pH, H2O2, polymyxin B, and lincomycin. Additionally, deleting brbI led to defective growth, cell division, and viability in Brucella suis S2. We then revealed the effect of brbI deletion on the physiological characteristics of Brucella suis S2 via integrated transcriptomic and proteomic analyses. The integrated analysis showed that brbI deletion significantly affected the expression of multiple genes at the mRNA and/or protein levels. Specifically, the affected divisome proteins, FtsB, FtsI, FtsL, and FtsQ, may be the molecular basis of the impaired cell division of the brbI mutant strain, and the extensively affected membrane proteins and transporter-associated proteins were consistent with the phenotype of the membrane properties’ alterations of the brbI mutant strain. In conclusion, our results revealed that BrBI is a bacterial cytoprotective protein involved in membrane homeostasis, cell division, and stress resistance in Brucella suis S2.
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影响因子：
5.2
作者：
Goolab S;Roth RL;van Heerden H;Crampton MC
通讯作者：
Crampton MC
影响因子：
1.2
作者：
Dajkovic, Alex;Lutkenhaus, Joe
通讯作者：
Lutkenhaus, Joe
影响因子：
16
作者：
Chae, HJ;Kim, HR;Reed, JC
通讯作者：
Reed, JC
DOI：
10.1084/jem.20030088
发表时间：
2003-08-18
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Celli J;de Chastellier C;Franchini DM;Pizarro-Cerda J;Moreno E;Gorvel JP
通讯作者：
Gorvel JP
影响因子：
5.6
作者：
Kihara, A;Akiyama, Y;Ito, K
通讯作者：
Ito, K